摘要
目的探讨Hog-MAPK信号通路在白念珠菌氟康唑耐药机制中的作用。方法通过Real-Time PCR、Western blot等方法比较白念珠菌氟康唑敏感株与耐药株Hog-MAPK信号通路相关的HOG1等基因的mRNA表达,以及磷酸化p38 MAPK蛋白表达的差异,并应用微量液基稀释法检测白念珠菌HOG1基因缺陷株及其原始亲代菌株/标准株对氟康唑MIC值的差异。结果白念珠菌氟康唑敏感株与耐药株之间Hog-MAPK信号通路相关基因mRNA表达和蛋白表达存在一定差异性,敏感株的表达在一定程度上低于耐药株,HOG1基因缺陷株对氟康唑更为敏感。结论白念珠菌氟康唑耐药性可能与Hog-MAPK信号通路中部分基因和蛋白表达有关,这些基因和蛋白表达的降低可能使耐药性发生改变。
Obejectives To investigate the role of Hog-MAPK signaling pathway in the fluconazole resistance mechanism of Candida albicans mechanism.Methods Using Real-Time PCR and Western blot to compare the expression of HOG1 gene and Phosphorylation p38 MAPK protein from Hog-MAPK signaling pathway in fluconazole sensitive strain and resistance strain,then dectect the fluconazole MIC of Candida albicans HOG1 mutant and its original parent strain/standard strain.Results The difference of the expression of Hog-MAPK signaling pathway related gene and protein is found between Candida albicans fluconazole sensitive strain and resistant strain,the expression of sensitive strain is less than resistant strain to a certain extent,and HOG1 mutant strain is more sensitive to fluconazole.Conclusion The fluconazole resistance mechanism of Candida albicans may be associated with some gene and protein from Hog-MAPK pathway.The decreased expression of these genes and proteins may cause change of resistance to fluconazole.
出处
《中国真菌学杂志》
CSCD
2015年第6期345-351,共7页
Chinese Journal of Mycology
基金
上海市科委自然科学基金(13ZR1437900)
