三种异喹啉型生物碱的微生物转化筛选

Strain screening on microbial transformation of three isoquinoline alkaloids

目的异喹啉型生物碱是生物碱的重要组成部分,药用价值高,本文拟筛选对3种四氢异喹啉型生物碱Z1（巴马亭）、Z2（番荔枝宁）、Z3（瑞枯灵）具有催化活性的微生物菌株,为后期通过微生物催化方法进行3种生物碱的结构修饰提供依据。方法以微生物菌株为酶催化剂,将作为底物的3种异喹啉型生物碱与18种微生物菌株共培养,3-5 d后乙酸乙酯萃取发酵液,通过薄层色谱法（TLC）对转化反应进行筛选;Z1和Z2两种底物转化能力均较强的菌株03-L-93-Ⅱ通过ITS区序列进行种属鉴定。结果经筛选的8株真菌、10株细菌中,03-L-93-Ⅱ、T002、T009、T016、T017具有转化Z1（巴马亭）的能力,T002、T009、T017、03-L-93-Ⅱ具有转化Z2（番荔枝宁）的能力,菌株L125-4对Z3（瑞枯灵）表现出微弱的转化活性。对菌株03-L-93-Ⅱ经分子生物学方法鉴定为Xylariales.Sp。结论微生物菌株作为催化剂对四氢异喹啉型生物碱进行结构改造是可行的。

Objective Isoquinoline alkaloids with high value in medicine were important structural type of alkaloids.The paper is to screen microbial strains which can catalyze isoquinoline alkaloids of palmatine（Z1）,xylopinine（Z2）,and reticuline（Z3）.Methods Isoquinoline alkaloids as the substrates were co-cultured with microbial strains which were catalysts for five to seven days,then the conversion reactions were identified by thin layer chromatography;strain 03-L-93-Ⅱwith high catalytic activity to Z1 and Z2 was identified by sequence of ITS region.Results In 8 strains of fungi and 10 strains of bacteria,strain 03-L-93-II,T002,T009,T016,T017 showed transformation activity of substrate Z1,strain T002,T009,T017,03-L-93-Ⅱ can transform Z2 to other compounds,while L125-4 showed weak catalytic ability of Z3.Strain 03-L-93-Ⅱwas confirmed as Xylariales.Sp by molecular method.Conclusion The microbial strains as catalysts to modify the structure of tetrahydroisoquinoline alkaloids were feasible and identified.