游泳及转笼训练对脑缺血再灌注大鼠神经功能及梗死灶Nogo-A、NgR表达的影响

Effects of different intensities of swimming and rolling cage on neurological function and expressions of Nogo-A and Ng R in cerebral ischemia reperfusion rats

目的:观察不同强度游泳及转笼训练对脑缺血再灌注大鼠神经轴突生长抑制剂-A(neurite outgrowth inhibitor-A,Nogo-A)、神经轴突生长抑制剂(Nogo receptor,Ng R)表达的影响。方法:将105只Wistar雄性大鼠随机均分为假手术组、对照组和游泳及转笼训练1、2、3组,后4组采用线栓法建立大脑中动脉闭塞实验动物模型,假手术组方法同上,但不阻塞大脑中动脉血流。在造模成功后24h进行游泳及转笼训练,游泳及转笼训练1、2、3组分别于训练后第3天、第7天、第14天行神经功能评分,后处死大鼠取脑免疫组化测定Nogo-A、Ng R阳性蛋白的表达。假手术组、对照组不进行训练。结果:游泳及转笼训练组Bederson行为学评分分别于训练后第3天、第7天、第14天较对照组降低(P<0.05或P<0.01),游泳及转笼训练1、2、3组行为学评分组间比较有显著性差异(P<0.05或P<0.01)。对照组脑梗死体积与假手术组比较明显增大(P<0.01),游泳及转笼训练1、2、3组大鼠与对照组比较,脑梗死体积明显减小(P<0.01)。对照组脑梗死组织中Nogo-A表达均明显高于假手术组(P<0.05)。训练后第3天,游泳及转笼训练3组Nogo-A表达明显低于对照组(P<0.05);第7天,游泳及转笼训练1、2、3组Nogo-A表达均明显低于对照组(P<0.05),游泳及转笼训练3组Nogo-A表达明显低于游泳及转笼训练2组(P<0.05);训练后第3天、第7天,游泳及转笼训练2、3组Ng R表达均明显低于对照组(P<0.05),游泳及转笼训练3组Ng R表达明显低于游泳及转笼训练2组(P<0.05);第14天,游泳及转笼训练3组Ng R表达明显低于对照组(P<0.05)及游泳和转笼训练2组(P<0.05)。结论:游泳及转笼训练能促进脑缺血再灌注大鼠神经功能恢复,且神经功能恢复与康复训练强度呈正相关,其对脑功能的保护作用可能与降低Nogo-A、Ng R表达有关。

Objective： To investigate the effects of swimming and rolling cage of different intensities on neurological function and expressions of neurite outgrowth inhibitor-A（Nogo-A） and Nogo receptor（Ng R） in cerebral ischemia reperfusion（CIR） rats.Method： One hundred and five male Wistar rats were randomly divided into sham group, control group and rehabilitation group 1,2,3. Intraluminal thread method was applied to make the left middle cerebral artery occlusion for 2h. After cerebral infarction for 24 h, rats in rehabilitation group 1 were trained by swimming once a day for 5min, rolling cage once a day for 20min; Rats in rehabilitation group 2 swimming twice a day for5min each time, rolling cage twice a day for 20 min each time; Rats in rehabilitation group 3 swimming twice a day for 10 min each time, rolling cage twice a day for 30 min each time; the behavioral score and expressions of Nogo- A and Ng R in brain tissue were observed on the 3th d, 7th d and 14 th d after training. The Sham group and control group had no training.Result： Bederson evaluation scores decreased significantly in rehabilitation group 1,2,3 compared with control group（P〈0.05 or P〈0.01） on the 3th d, 7th d and 14 th d after training, it were significantly different among rehabilitation group 1,2,3. The infarction volume of control group were significantly different compared with sham group（P〈0.01）. The infarction volume of rehabilitation group 1,2,3 decreased significantly compared with control group（P〈0.01）. Expression of Nogo- A increased in control group compared with sham group（P〈0.05）, expression of Nogo- A decreased in rehabilitation group 3 compared with control group（P〈0.05） on the 3th d, and decreased in rehabilitation group 1,2,3 compared with control group（P〈0.05） on the7 th d, Nogo-A of rehabilitation group 3 decreased compared with rehabilitation group 2（P〈0.05）. Expression of Ng R decreased in rehabilitation group 2,3 compared with control group（P〈0.05） on the 3th d and 7th d,Ng R of rehabilitation group 3 decreased compared with rehabilitation group 2（P〈0.05） on the 3th d and 7th d, Ng R of rehabilitation group 3 decreased compared with control group（P〈0.05） and the rehabilitation group2（P〈0.05） on the 14 th d.Conclusion： The swimming and rolling cage of different intensities can improve the neurological function in cerebral ischemia reperfusion rats, and the intensity was positively correlated with neurologic function recovery.The swimming and rolling cage of different intensities may decrease the expressions of Nogo-A and Ng R.