摘要
目的回顾性研究人脐带间充质干细胞(UC—MSC)治疗乙型肝炎相关性肝硬化失代偿期(HBV—D-LC)患者的疗效和安全.陛。方法入组HBV—D—LC患者60例,给予综合治疗联合静脉输注UC—MSC,UC—MSC细胞剂量为(0.5~1.0)×10^6个/kg,每月1次,共输注3次;按照1:2原则匹配120例HBV-D—LC患者作为对照组,给予综合治疗,匹配因素包括年龄、性别、诊断和随访时间。收集并记录观察组输注UC—MSC前后及对照组随访期间的血清生物化学指标、血常规、腹部B型超声、CT和(或)MRI检查、Child-Pugh评分及不良反应等数据。计量资料比较采用两独立样本t检验;计数资料比较采用x^2检验。结果与对照组相比,观察组在回输UC—MSC后12周以及24周胆碱酯酶和白细胞水平显著升高垆〈0.05);12周、24周和96周球蛋白水平明显升高(P〈0.05);12周、24周和48周的碱性磷酸酶水平有明显升高(P〈0.05);Child—Pugh评分在12周开始降低(尸〈0.05)。但两组问丙氨酸转氨酶、天冬氨酸转氨酶、总胆红素、白蛋白、总胆固醇、甘油三酯、肾脏功能指标和凝血酶原活动度等指标变化差异无统计学意义妒〉0.05)。结论人UC—MSC治疗可有效改善HBV—D—LC患者的胆碱酯酶和白细胞水平,降低Child-Pugh评分,且安全性良好,具有一定的临床应用前景。
Objective To retrospectively investigate the efficacy of human umbilical cord-derived mesenchymal stem cells (UC-MSC) as clinical treatment for HBV-related decompensated liver cirrhosis (HBV- DLC)oD. Methods Sixty patients with HBV-DLC were given standard medical treatment combined with a 3-month regimen of UC-MSC at a dose of 0.5-1.0×10^6 cells/kg/month. Another group of patients with HBV-DLC (n=120; control group) that was matched (2:1) to the case group by age, sex, diagnosis, and follow-up period was given the standard medical treatment only. We reviewed all patients' data of biochemical tests, imaging examinations, Child-Pugh scores, and adverse reactions. Comparisons of continuous data between the two groups were made by independent-sample t-test, and comparisons of categorical data were made by chi-square test. Results Compared with the control group, the group that received the combination UC-MSC treatment showed a significant rise in cholinesterase, globulin and alkaline phosphatase, and reduced Child-Pugh scores during the follow-up period.However, there was no significant difference between the groups of patients for levels of alanine transaminase, total bilimbin, albumin, total cholesterol, or prothrombin activity. Conclusions Addition of the UC-MSC treatment to the standard therapy could help to improve liver function in patients with HBV-DLC.
出处
《中华肝脏病杂志》
CAS
CSCD
北大核心
2016年第1期51-55,共5页
Chinese Journal of Hepatology
基金
国家高技术研究发展计划(863计划)(ss2013AA020102)
国家自然科学基金(81571567)
关键词
肝硬化
失代偿性
肝炎
乙型
脐带间充质干细胞
胆碱酯酶
Decompensated liver cirrhosis
Hepatitis B
Umbilical cord derived-mesenchymal stemcell
Cholinestemse