微波辅助提取暴马丁香中紫丁香苷和橄榄苦苷的工艺研究

Optimization of Microwave-assisted Extraction of Syringin and Oleuropein from Syringa reticulata

主要采用微波辅助提取法对暴马丁香中紫丁香苷和橄榄苦苷两种重要活性成分的同时提取工艺进行了研究,通过Box-Behnken响应面法分析建立二次多项式数学模型,优化提取工艺。结果表明,工艺所及因素对紫丁香苷和橄榄苦苷两种物质总得率影响大小的顺序是乙醇体积分数>微波时间>微波功率,提取工艺最佳参数条件为:浸泡时间1 h,45%乙醇溶液,料液比例1∶20 g·m L^(-1),微波功率600 W,提取时间5 min。在此最佳条件下,紫丁香苷和橄榄苦苷总得率5.28%±0.102%。该工艺高效省时,投入一批原料,即可同时提取其中的紫丁香苷和橄榄苦苷,有利于暴马丁香资源的综合加工利用。

We used microwave-assisted extraction to extract syringin and oleuropein from Syringa reticulate, and optimized the extraction process by the Box-Behnken response surface method. The order of the effect of factors on the yield of syringin and oleuropein was the concentration of ethanol 〉 the microwave time 〉 microwave power. The optimum conditions for the extraction were as follows ： soaking time of 1 h, ethanol concentration of 45 % , ratio of solid to liquid of 1：20 g · mL^-1, microwave output power of 600 W, and extraction time of 5 min. With the optimum extraction conditions, the yield of syringin and oleuropein from Syringa reticulate was 5.28% ± 0. 102%. The microwave-assisted extraction was efficient and rapid for extraction syringin and oleuropein from Syringa reticulate, and was benefit for the comprehensive utilization of Syringa reticulate.