接触性检材502胶熏显后手印脱落细胞的DNA提取

DNA Extraction of Cast-off Cells of Fingerprints from 502 Glue Fumigated Con-tact Samples

目的建立接触性检材中手印定位、检材转移、DNA提取纯化的方法。方法 66例接触性检材经502胶熏显,手印接触部位明确后,分别采用普通擦拭法及丙酮擦拭法进行检材上手印脱落细胞的转移,并使用超微量磁珠法试剂盒提取纯化手印脱落细胞DNA,扩增后分析结果。结果用丙酮擦拭法得到的33例检材有30份获得了较好的STR分型结果,峰值范围为1 000～4 000 RFU,峰型均匀;而普通擦拭法很难获得理想的STR分型。结论通过丙酮擦拭法转移502胶熏显的手印脱落细胞,STR分型效果更优良,可应用于法庭科学实践。

Objective To establish a method of fingerprint position,sample transfer and fingerprint DNA extraction in contact samples.Methods Sixty-six cases were visualized by 502 glue fingerprint fumigation.Two methods,ordinary wipe and acetone wipe,were used to transfer cast-off cells of fingerprints from testing samples,respectively.DNA was extracted and purified by ultramicro magnetic bead kit.The data was resolved on genetic analysis after amplification.Results In 33 samples,30 samples got better STR analysis by acetone wipe method.The peak range was 1 000-4 000 RFU and peak shapes were equable.It was hard to get ideal STR typing by ordinary wipe method.Conclusion The samples are visualized by 502 glue fingerprint fumigation and the case-off cells are transferred by acetone wipe method.The method shows better STR analysis result,which might be a better method for forensic science practice.