肾炎消白颗粒对阿霉素肾病大鼠α3β lintegrin mRNA的影响

The Influence of Nephrms Xiaobaikeli on α3β Lintegrin of Rats with Adriamycin Nephrosis

目的:观察肾炎消白颗粒对阿霉素肾病大鼠足细胞-基底膜连接膜蛋白α3βlintegrin mRNA的影响。方法:将100只Wistar大鼠随机分正常对照组、模型对照组、肾炎消白颗粒高剂量组、肾炎消白颗粒低剂量组、洛汀新组,每组各20只。经尾静脉一次性注入阿霉素6.5 mg·kg^(-1),建立阿霉素肾病大鼠模型。分别采用肾炎消白颗粒和洛汀新进行治疗,7周末处死大鼠,取大鼠右肾皮质,RT-PCR检测α3βlintegrin mRNA表达。结果:7周末阿霉素大鼠模型组α3β lintegrin mRNA表达明显下调,肾炎消白颗粒、洛汀新组α3β lintegrin mRNA的表达明显上调,其中,肾炎消白颗粒高剂量组的作用优于洛汀新组,差异具有统计学意义(P<0.05)。结论:肾炎消白颗粒治疗肾小球肾炎蛋白尿的机制之一可能是通过上调足细胞α3β lintegrin mRNA的表达,减少了足细胞的剥离与脱落,维护了正常的肾小球滤过屏障。

Objective： To observe the clinical curative effect of Nephrms Xiaobaikeli（ NXBKL） on α3β lintegrin of rats with Adriamycin nephrosis. Methods： One hundred Wistar rats were randomly divided into the normal control group,the model control group,the high-dosage NXBKL group,the low-dosage NXBKL group,and the Lotensin group,with 20 in each group. 6. 5 mg / kg of adriamycin was one-off injected via tail vein to establish the model of rats with adriamycin nephrosis. The rats were treated with NXBKL and Lotensin respectively and then killed at the end of the 7th week. The right kidney cortex of rats was taken to detect α3β lintegrin mRNA expression by RT-PCR method. Results： At the end of the 7th week,α3β lintegrin mRNA expression of the model of rats with adriamycin nephrosis clearly reduced,while those of the NXBKL groups and the Lotensin group clearly increased. The function of the high-dosage NXBKL group was better than that of the Lotensin group. And the difference between them had statistical significance（ P〈0. 05）. Conclusion： One of the mechanisms of NXBKL in treating glomerulonephritis proteinuria may exist in increasing the sertoli cell α3β lintegrin α3β lintegrin mRNA expression,and reducing the sertoli cell detachment and loss in order to maintain the normal glomerular filtration barrier.