反高效液相色谱法测定地骨皮中大黄素、大黄素甲醚实验研究

The Research into the Experiment of the Content Determination of Emodin and Emodin Monomethyl Ether in Digupi( Cortex Lycii) by Reversed Phase High Performance Liquid Chromatography

目的:观察反高效液相色谱法测定地骨皮中大黄素、大黄素甲醚的含量情况。方法:采用Agela C18色谱柱,以甲醇-0.1%磷酸水溶液(85∶15)为流动相,流速1.0 m L·min^(-1),检测波长254 nm,柱温为25℃,对地骨皮中大黄素,大黄素甲醚成分含量进行测定。结果:该检测方法在(0.1~25.0)mg·L-1浓度范围内线性关系良好,溶液稳定性、重复性及加样回收率良好。结论:反高效液相色谱法操作简便、准确、重复性好,可以用于地骨皮药材中大黄素、大黄素甲醚含量测定,以控制其质量。

Objective： To observe the content determination of emodin and emodin monomethyl ether in Digupi（ Cortex Lycii） by reversed phase high performance liquid chromatography. Methods： By using Agela C18 chromatographic column with methanol,0. 1% phosphoric acid aqueous solution（ 85∶15） as mobile phase,flow rate of 1. 0 m I / min,detection wavelength of 254 nm,column temperature is 25 ℃ to detect the content of emodin and emodin monomethyl ether in Digupi（ Cortex Lycii）,thus the content determination method was established. Results： Within the concentration range of（ 0. 1- 25. 0） mg·L-^1,this determination method had good linear relationship,and excellent solution stability,repeatability and the added sample recovery rate. Conclusion：In terms of reversed phase high performance liquid chromatography,its operation is simple,accurate,reproducible,and can be used for the content determination of emodin and emodin monomethyl ether in Digupi（ Cortex Lycii） to control their quality.