摘要
用有机溶剂萃取和树脂吸附纯化醇酶法制大豆油废液中的异黄酮,用高效液相色谱检测纯化的异黄酮的纯度。以人乳腺癌细胞系MCF-7为模型,检测纯化的大豆异黄酮对MCF-7增殖的影响。结果表明,萃取法粗提的最佳萃取溶剂为乙酸乙酯与正丁醇(1∶1)的混合液,异黄酮提取率为70.47%,纯度为26.45%;树脂吸附纯化的最佳条件为:树脂型号,LSA-8;上样浓度,0.8 mg/m L;上样流速,0.5 BV/h;洗脱液,75%的乙醇;洗脱流速,6 BV/h;洗脱体积,1.5倍上样体积,异黄酮提取率为83.71%,纯度为72.09%。抗肿瘤实验表明,大豆异黄酮浓度达到100 mg/L时,能显著(p<0.01)抑制MCF-7细胞的增殖,其作用有一定的剂量依赖性。
The isoflavone was purificated from byproducts of enzyme- assisted alcohol leaching soybean oil by organic solvent extraction and resin adsorption. The purity of isoflavone was investigated by high performance liquid chromatography（ HPLC）. The human breast cancer cell line MCF- 7 was used as the model cells and the affect of soybean isoflavone on the proliferation of MCF-7 cells were studied.The result showed that the optimum extraction solvent for purification of soybean isoflavone was ethyl acetate and n- butyl alcohol mixture（ 1∶ 1） and the extraction rate and purity of isoflavone were 70.47% and 26.45%,respectively. The optimum conditions of resin adsorption for extraction of isoflavone were as follows： the adsorption resin was LSA-8,the concentration and flow rate of the sample were 0.8 mg / m L and 0.5 BV / h,respectively,the eluent solution was 75% ethanol and the flow rate and volume of eluent solution were 6 BV / h and 1.5 times of the sample volume,respectively.The extraction rate and purity of soybean isoflavone under the optimal condition of resin adsorption extraction were 83.71% and72.09%,respectively.The MCF- 7 cell experiments suggested that when the concentration of soybean isoflavone was up to 100 mg / L,it could significantly inhibit the proliferation of human breast cancer cell. And the anticancer function of soybean isoflavone was concentration- dependent.
出处
《食品工业科技》
CAS
CSCD
北大核心
2016年第1期104-109,共6页
Science and Technology of Food Industry
