八月瓜果皮果胶提取工艺优化及其理化特性研究

Optimization of extraction conditions and analysis of physicochemical property of pectin from Akebia trifoliata peel

以八月瓜果皮为原料,优化酸提醇沉法提取其果胶的工艺,并对果胶酯化度、半乳糖醛酸含量进行测定。首先采用单因素实验探讨了料液比、p H、提取时间、提取温度等因素对果胶得率的影响,再通过正交实验优化提取工艺,最后对果胶的酯化度、半乳糖醛酸进行分析。结果表明,酸提醇沉法提取八月瓜果胶的最佳工艺条件为液料比1∶20(g/m L)、p H1.5、提取时间120 min、提取温度90℃,此条件下得率达12.15%;八月瓜果皮果胶的酯化度为81.94%,属于高酯果胶,其半乳糖醛酸含量为83.17%,符合GB 25533-2010对果胶半乳糖醛酸含量的要求。

Using Akebia trifoliata peel as the material,optimization of the acid extracting and alcohol precipitation process to extract pectin from Akebia trifoliate peel,and the degree of esterification and galacturonic acid of its physicochemical property were investigated. First of all,the effects of solid to liquid ratio,p H,extracting temperature and extracting time on the pectin yield were discussed by the single factor tests. And then,the orthogonal experiment was used to optimize the extraction conditions,and the degree of esterification and galacturonic acid were analyzed at last. The results showed that the optimum conditions of acid extraction and alcohol precipitation process of extracting pectin from Akebia trifoliate peel were as follows： solid to liquid- ratio1 ∶ 20（ g / m L）,p H1.5,extracting temperature 90 ℃,extracting time 120 min,and the extraction yield was 12.15 %under these conditions.The pectin from Akebia trifoliata peel was a kind of high methoxyl pectin,its degree of esterification was 81.94%,and its galacturonic acid was 83.17%,meet GB 25533- 2010 of galacturonic acid content requirements.