微波消解-电感耦合等离子体质谱法同时测定椴树蜂蜜中23种元素

Simultaneous determination of 23 elements in linden honey by microwave digestion- inductively coupled plasma mass spectrometry method

椴树蜂蜜是我国东北地区特色蜜种,为了解椴树蜂蜜中元素含量分布,本文建立了微波消解-电感耦合等离子体质谱（ICP-MS）同时测定椴树蜂蜜中B,Na,Mg,P,K,Ca,V,Cr,Mn,Fe,Co,Ni,Cu,Zn,Ga,As,Rb,Sr,Mo,Cd,Ba,Pb和U等23种元素的分析方法。结果表明,该方法加标回收率（n=6）在86.5%~109.8%之间,选择国家标准物质菠菜（GBW10015）和茶叶（GBW10016）验证方法的可靠性,待测元素测定值基本在标准值允许范围内,日内相对标准偏差（n=3）在0.3%~8.7%之间,日间相对标准偏差（n=5）在1.2%~10.4%之间。应用该方法测定了吉林省不同采样点的10个椴树蜂蜜,结果发现K的含量最高,As,Cd和Pb含量均符合蜂蜜中相关限量标准要求。根据测定结果,建立了吉林省椴树蜂蜜中元素指纹图谱,发现不同产地的10个椴树蜂蜜样品中元素分布具有共同特征,可为椴树蜂蜜蜜种鉴别提供判别依据。

The determination of 23 elements including B,Na,Mg,P,K,Ca,V,Cr,Mn,Fe,Co,Ni,Cu,Zn,Ga,As,Rb,Sr,Mo,Cd,Ba,Pb and U in linden honey,which is characteristic of honey in northeast China,was studied by inductively coupled plasma mass spectrometry（ ICP- MS） using microwave digestion for sample preparation. The recoveries for spiked honey samples were in the range of 86.5% ~109.8%.The method was validated by analyzing the National Standard Reference Material of Spinach（ GBW10015） and Tea（ GBW10016）,which obtained the relative standard deviation values of repeatability（ n = 3） and reproducibility（ n = 5） were 0.3% ~ 8.7% and 1.2% ~10.4%,respectively.Ten linden honey samples collected in different sites from Jilin province were studied by this method.Among the 23 elements,K was abundant in linden honey,As,Cd and Pb were lower than relevant limit requirements. Furthermore,the element fingerprint of linden honey from Jilin province was established,which showed that various samples presented common features and could provide a basis for the identification of linden honey.