氨基磁性纳米粒子对α-胰凝乳蛋白酶固定过程的研究

Process of α-chymotrypsin Immobilized on Amino-functionalized Magnetic Nanoparticles: Research

采用分子自组装技术制备氨基功能化磁性纳米粒子,并通过碳二亚胺偶联剂将α-胰凝乳蛋白酶固定在其表面.利用红外光谱及热失重分析等方法表征固定酶的纳米粒子,研究了固定过程中酶浓度和反应时间对载酶量的影响,得到最佳固定条件.在固定过程用Langmuir等温式拟合优于用Freundlich等温式,功能化纳米粒子的载酶量达到127 mg·g-1,与一级反应动力学模型相比,二级反应动力学模型能够更好地拟合该固定过程.

In this paper, a novel amino-functionalized Fe_3O_4 nanoparticles is presented by molecular self-assembly and bound to α-chymotrypsin in presence of carbodiimide. The immobilized α-chymotrypsin NPs are characterized by IR and TGA. The effect of parameters such as the initial concentration and reaction time on the process of immobilization is investigated. The Langmuir isotherm model is in agreement with the experimental data better than Freundlich isotherm model in the process of immobilization. The binding capacity of functional nanoparticles is measured for being 127 mg·g^-1. The immobilization kinetic data correlate better with the second order equation than with the first order equation.