血细胞分析4个项目参考区间行业标准在汉、维族人群的适用性验证

Verification of the clinical applicability of the published standard reference interval:a retrospective study based on health examination results of Han and Uygur nationality

目的 应用乌鲁木齐汉族和维吾尔族（以下简称维族）体检健康人群检测结果,验证白细胞（WBC）、红细胞（RBC）、血小板（PLT）和血红蛋白（Hb）参考区间行业标准（WS/T405-2012）的适用性。方法 采用回顾性研究收集2013年8月至2015年1月在新疆生产建设兵团医院体检中心进行团体健康体检的汉族和维族20-79岁成年人体验结果,利用体检软件筛选出汉族和维族健康参考个体共9 307名,其中汉族8 506名,维族801名。分别统计汉族和维族健康参考个体WBC、RBC、PLT和Hb检测结果的中间95%CI,计算汉族和维族参考个体各项目检测结果超出参考区间行业标准的例数所占比例。验证的判断标准：超出例数低于10.00%则通过验证。结果 正态性检验结果表明汉族和维族体检健康参考个体各项目的统计数据均呈偏态分布。汉族和维族参考个体4个项目检测结果中间95%区间：WBC汉族（3.6-9.6）×10^9/L,维族（3.8-9.9）×10^9/L;RBC汉族男性（4.3-5.9）×10^12/L和女性（3.9-5.2）×10^12/L,维族男性（4.4-5.8）×10^12/L和女性（3.9-5.2）×10^12/L;PLT汉族（130-351）×10^9/L,维族（145-370）×10^9/L;Hb汉族男性（133-178）g/L和女性（111-153）g/L,维族男性（133-174）g/L和女性（110-152）g/L。各项目检测结果在同一民族不同性别间的差异均有统计学意义（均P〈0.01）。汉族和维族各项目检测结果超出行业标准参考区间的参考个体例数均在10.00%以内。结论 WBC、RBC、PLT和Hb的参考区间行业标准适用于本实验室检测的汉族和维族人群。

Objective To verify the clinical applicability of the published standard intervals （WS/T402-2012） for WBC, RBC,PLT and Hb Based on the health examination results of Han and Uygur populations in Urumqi. Methods This was a retrospective study, The results of WBC, RBC, PLT and Hb from health examination individuals of Han and Uygur populations from August 2013 to January 2015 were collected,9 307 health subjects age range from 20 to 79 years of the two nationalities were chosen using health examination information system. The percents of health subjects failed falling in the published standard interval were calculated to verify the judgment criterion,fail falling rate〈10.00% was regard as qualified. Results The test of normality revealed that the Han and Uygur s results of all verified items were skewed distributions. The 2.5% and 97.5% percentiles of the results of two nationalities：WBC Han（3.6-9.6）×10^9/L,Uygur（3.8-9.9） ×10^9/L;RBC Han Male （4.3-5.9）×10^12/L and Female （3.9-5.2） ×10^12/L,Uygur Male （4.4-5.8）×10^12/L and Female （3.9-5.2）×10^12/L;PLT Han（130-351） ×10^9/L, Uygur（145-370） ×10^9/L） ,and Hb Han：Male （133-178）g/L and Female （111-153）g/L,Uygur： Male （133-174） g/L and Female （110- 152）g/L. The significant differences of each testing item were found in the different sex from the same nationality（P〈 0.01）. The percents of health subjects failed falling in the published standard interval were〈10.00 % in both Han and Uygur nationality. Conclusion The published standard reference intervals for WBC,RBC,PLT and Hb are applicable to our laboratory for the detection of Han and Uygur populations.