新疆汉族、维吾尔族丙型肝炎感染者HCV基因型研究

Hepatitis C virus genotyping of Han and Uygur patients in Xinjiang Uygur autonomous region

目的了解新疆不同地区汉族和维吾尔族(以下简称维族)丙型肝炎感染者丙型肝炎病毒(HCV)基因分型的特征,为新疆丙型肝炎的诊断和治疗提供依据。方法对380份汉族和维族丙型肝炎感染者血清样本先进行荧光定量PCR检测病毒载量,载量大于1×103 copies/mL的再采用PCR-反向点杂交法进行HCV基因分型。结果基因分型确定355份样本,成功分型率为93.4%,汉族和维族基因型检出率1b型分别为59.91%、69.92%,2a型分别为30.17%、12.20%,3a型分别为5.60%、8.13%,3b型分别为3.88%、8.94%。新疆乌鲁木齐和其他地区丙型肝炎感染者中,维族、汉族病例分布差异有统计学意义(P<0.05),且乌鲁木齐和其他地区男性、女性病例分布差异有统计学意义(P<0.05)。乌鲁木齐地区病例中,维族、汉族男性感染者2a型和女性感染者1b、3b型差异均有统计学意义(P<0.05)。新疆其他地区,仅2a型男性维族、汉族感染者发病有差异有统计学意义(P<0.05),其他基因型差异无统计学意义(P>0.05)。结论新疆地区汉族和维族丙型肝炎感染者HCV基因型分布与国内大多数地区不同,汉族以1b、2a型为主,而维族以1b型为主,其次是2a、3a、3b型。

Objective To investigate the genotyping characteristics of Han and Uygur patients with hepatitis C virus（HCV） in Urumqi and other area of Xinjiang,and provide information for diagnosis and treatment. Methods Totally 380 samples of Han and Uygur patients virus load were detected by real-time PCR, with the load greater than 1 ×10^3 copies/mL, HCV genotyping was carried out by PCR-reverse dot blot hybridization. Results A total of 355 samples（93.4%） was genotyped successful. Type 1b of Han and Uygun were 59.91% ,69.92%,type 2a were 30. 17% ,12.20% ,type 3a were 5.60%,8, 13% and type 3b were 3.88%, 8.94%. In Urumqi and other areas, significant difference of patient distribution, male and female were found between Han and Uygur patients（all P〈0.05）,In Urumqi,type 2a had significant difference between Uygur and Han male patients, type 1b,3b had significant difference in female patients（P〈0. 05）. In other areas except Urumqi,type 2a had significant difference between Uygur and Han man（P〈0.05）,other genotypes were not found difference（P〈0.05）. Conclusion HCV genotyping of Uygur and Han patients in Xinjiang is different with the majority areas in China,type 1b and 2a are the main infectious virus in Han,and type lb is the main infectious virus in Uygur, followed by type 2a, 3a, 3b.