IL-6在儿童慢性鼻窦炎中的表达及其意义

Expression and significance of interleukin-6 in children with chronic rhinosinusitis

目的研究白细胞介素-6(IL-6)在儿童慢性鼻窦炎(CRS)患儿外周血及腺样体中的表达情况,探讨IL-6在CRS患儿发生、发展中的作用机制。方法收集CRS伴有腺样体肥大患儿95例(CRS组),变应性鼻炎伴有腺样体肥大患儿70例(变应性鼻炎组),单纯腺样体肥大患儿51例(对照组),采用ELISA法检测3组患儿外周血中IL-6的水平;免疫组织化学染色观察3组患儿腺样体组织中IL-6的分布及表达情况。结果对照组外周血IL-6的水平为(33.6±6.9)pg/mL,变应性鼻炎组为(35.9±5.6)pg/mL,CRS组(49.8±8.3)pg/mL,CRS组与变应性鼻炎组间差异有统计学意义(P<0.05);CRS组与对照组间差异有统计学意义(P<0.05),变应性鼻炎组与对照组间差异无统计学意义(P>0.05)。腺样体组织中IL-6主要分布在浸润的炎性细胞、成纤维细胞,少量分布在间质,CRS组与变应性鼻炎组间差异有统计学意义(P<0.05),CRS组与对照组间差异有统计学意义(P<0.05),变应性鼻炎组与对照组间差异无统计学意义(P>0.05)。结论儿童CRS外周血中及局部组织中IL-6表达均明显高于不伴CRS的儿童,提示IL-6可能在儿童CRS的发生发展中起着重要作用。

Objective To evaluate the expression of interleukin-6（IL-6） on peripheral blood and adenoid in children with chronic rhinosinusitis （CRS） and adenoidal hypertrophy, to investigate the mechanism of IL-6 on the occurrence and development of CRS. Methods A total of 216 cases of hypertrophic adenoids were collected,including 95 CRS children with hypertrophic adenoid （CRS group）, 70 children with allergic rhinitis and hypertrophic adenoid （AR group）and 51 children with hypertrophic adenoid （control group）. The plasma IL-6 concentrations were detected by ELISA,and the expression and distribution of IL-6 in adenoid tissue was detected by immunohistochemistry. Results The plasma IL-6 level in CRS group was （49.8±8.3）pg/mL,significantly higher than that of in control group （33.6±6.9）pg/mL and in AR group （35.9 ± 5.6）pg/mL, differences had statistic significance （P〈0. 05）. However, there was no statistically significant difference between AR group and control group. Meanwhile,the presence of IL-6 in adenoid tissue was mainly seen m inflammation cells and fibroblasts while less in mesenchymal ceils, there were statistical differences between CRS group and control group/AR group （P〈0.05）, but no statistical difference between AP group and control group（P〉0.05）. Conclusion Among children with CRS,expression of IL-6 whether in peri-plasma or in focal tissue was higher than those without CRS, demonstrating that IL-6 may play an important role in the pathogenesis and developemnt of CRS in children.