rDNA-ITS 序列测定等三种方法鉴定假丝酵母菌结果比较

Comparison of CHROMagar Chromogenic Medium,API 20C AUX,and r DNA-ITS Sequence Analysis Methods in Candida Identification

目的通过三种假丝酵母菌鉴定方法的比较,为临床选择假丝酵母菌的鉴定方法提供参考。方法用科玛嘉显色培养基、API 20C AUX生化系统和rRNA-ITS序列测定分析鉴定住院患者口腔培养出的198株假丝酵母菌,比较3种鉴定方法的结果。结果 API 20C AUX生化系统与rDNA-ITS序列测定分析鉴定结果一致,科玛嘉显色培养基与API 20C AUX生化系统和rDNA-ITS序列测定分析鉴定的符合率白假丝酵母菌为97.84%,热带假丝酵母菌的符合率为93.33%,光滑假丝酵母菌和克柔假丝酵母菌的符合率分别为90.91%、88.89%,其他假丝酵母菌科玛嘉不能鉴定。结论 API 20C AUX生化系统与rDNA-ITS序列测定分析鉴定假丝酵母菌的结果有较好的一致性,rDNA-ITS序列测定分析鉴定假丝酵母菌有望成为假丝酵母菌鉴定较好的方法。科玛嘉显色培养基方法可以简便有效的鉴定最常见的4种假丝酵母菌。

Objective To study the results of CHROMagar chromogenic medium, API 20C AUX biochemistry system and rDNA-ITS sequence analysis in identifying candida, and then compare their results. Methods Used CHROMagar ehromogenie medium, API 20C AUX biochemistry system and rDNA-ITS sequence analysis methods to identifieate 198 eandidia species which were isolated from oral cavity of in-patients, then compared the identification result of these three methods. Results The identified results of API 20C AUX biochemistry system and rDNA-ITS sequence analysis methods are same. Identification of Candida albicans by CHROMagar chromogenic medium is of 97.84% consistency of API 20C AUX biochemistry system and rDNA-ITS sequence analysis methods, whereas 93.33%, 90. 91% and 88.89% for Candida. tropicalis, Candida glabrata and Candida krusei respectively. CHROMagar chromogenic medium can not identify other eandida species. Conclusion API 20C AUX biochemistry system and rDNA-ITS sequence analysis methods can accurately identify all Candida spices. The CHROMAgar Candida medium is an easy and economic method for presumptive identification of the most commonly encountered Candida spp.