吲哚乙酸/辣根过氧化物酶对SACC-83细胞增殖和凋亡的影响

Effects of IAA/HRP on the proliferation and apoptosis of SACC-83 cells

目的:探讨植物激素中吲哚乙酸/辣根过氧化物酶(IAA/HRP)对人涎腺腺样囊性癌SACC-83细胞体外增殖和凋亡的影响及机制。方法:采用CCK-8比色法检测IAA/HRP对人涎腺腺样囊性癌SACC-83增殖的抑制作用,应用实时荧光定量PCR及Western blot检测凋亡相关基因Caspase-3和Livinα基因mRNA及蛋白表达水平的改变。结果:IAA/HRP对SACC-83细胞增殖有明显的抑制作用,且呈时间和浓度依赖性(P<0.05);不同浓度的IAA/HRP(60、100μmol/L IAA+1.2 mg/L HRP)可上调Caspase-3 mRNA及蛋白的表达(P<0.05),下调LivinαmRNA及蛋白的表达(P<0.05)。结论:IAA/HRP可抑制SACC-83增殖、诱导细胞凋亡,其作用机制是通过调节Caspase-3和Livinα的表达而实现的。

Objective：To study the effect of IAA/HRP on the proliferation and apoptosis of human SACC-83 cells in vitro,and its molecular mechanism.Method：The rate of proliferation inhibition was determined by CCK-8assay,the apoptosis-related gene Caspase-3and LivinαmRNA and protein expression levels were measured by realtime PCR and Western blot.Result：IAA/HRP could inhibit proliferation of SACC-83,which depending on the time and dosage（P0.05）;can up-regulate the Caspase-3mRNA and protein expression levels and reduction of the mRNA and protein of the Livinαexpression,（both P0.05）.Conclusion：IAA/HRP can inhibit the proliferation and induce the apoptosis of SACC-83 cells,which may due to its regulation of the expression of Caspase-3and LivinαmRNA expression.