副猪嗜血杆菌分型血清制备及其四川地区流行病学

Preparation of antisera to different serotype of Haemophilus parasuisand application in epidemiology study of Sichuan province

从澳大利亚昆士兰州动物研究所购买副猪嗜血杆菌(Haemophilus parasuis,HPs)15个血清型的标准菌株制备免疫原,采用皮下注射与耳缘静脉注射免疫原的方式免疫健康雄性家兔,免疫结束后采集心血并分离血清,对血清进行特异性与抗体效价检测,结果显示血清特异性良好,抗体效价均达到1∶8以上,用于田间分离株的血清型鉴定有良好效果。本实验室于2014年从四川22个市区80个猪场中共采集498份病料,498份病料中共检出80株HPs。采用琼扩试验对80株HPS进行血清型鉴定,所分离的80株HPs中血清4型占28.75%、5型占35.00%、12型占6.25%、13型占7.50%、15型占1.25%、不能分型的菌株占21.25%,结果表明:血清型4、5、12、13为四川地区主要流行的血清型。

Immunogen was prepared from the 15 serovars of Haemophilus parasuis standard strain purchased from Animal Research Institute of Australia Queensland, which was used to immune male rabbits by subcutaneous and ear vein injection. After the immune procedure, the blood serum was separated by the hemospasia from the heart,and detected for serum specific and antibody ti- ters. The results showed that the serum specific was good,and the serum antibody titers was grea- ter than 1 ： 8,which indicated the separated serum could be used for serovar identification. Four hundreds and ninety eight pathological materials were collected from 80 pig farms of 22 cities of Sichuan province by our laboratory, from which 80 HPS was identified. The serovar of 80 HPS was identified by agar diffusion test, which showed that serovar 4 （28. 75%）, 5 （35. 00%）, 12（6.25%）,13（7. 50%）,15（1.25%） and non-typable strains （21. 25%） from the 80 filed iso- lates. These results indicated that serovar 4,5,12 and 13 were the predominant serovar in the source areas of the isolates,which could be provided a reliable theoretical basis for the detection of the prevalence of HPS from Sichuan Province and screening of the vaccine strains.