番鸭肠道乳酸菌的分离鉴定及抑菌性能

Isolation and identification of Lactobacillus in Muscovy duck intestine and its bacteriostatic action

通过革兰染色、菌落形态观察、生化分析鉴定、牛津杯抑菌试验、Caco-2细胞黏附试验对分离到的6株乳酸产生菌进行研究。结果显示:经16SrDNA序列测定,比对分析6株乳酸产生菌与鼠李糖乳杆菌、乳酸乳球菌乳亚种、唾液乳杆菌、乳链球菌、哥伦比亚肠球菌和鼠乳杆菌高度同源。6株乳酸菌抑菌试验检测显示:对大肠杆菌、沙门菌、蜡样芽胞杆菌和金黄色葡萄球菌具有很强的抑菌效应,以CM3菌株的抑菌效果最强。同时,这6株乳酸菌对Caco-2细胞均有一定的黏附能力。结果表明:6株番鸭肠道乳酸产生菌能够黏附于肠道模型Caco-2细胞上并具有较强的抑菌活性,但其黏附性能及保护机制还有待进一步研究。

The purpose of this study isolated the Muscovy duck intestinal Lactobacillus by the an- aerobic separation technology. 6 strains of isolated Lactobacillus were marked CM1, CM2, CM3, CM4,CM5 and CM6. They were identified as gram-positive bacterium via Gram strain. Subse- quently, colonial morphology observation,biochemical identification and 16S rDNA sequence anal- ysis showed that 6 strain of isolated Lactobacillus was highly homology with Lactobacillus rham- nosus , Lactococcus lactis sub sp. Lactis,Lactobacillus salivarius , Streptococcus lactis , Enterococcus columbae and Lactobacillus rnurinus. In vitro, bacteriostatic test of six isolates showed Lactoba- cillus was highly bacteriostatie effect on E. coli, Salmonella, and Bacillus cereus and Staphylococ- cus aureus. Thereinto, the CMa has the strongest comprehensive bacteriostatic effect. All the 6 strains had a adherence ability. These results suggest that Muscovy ducks intestinal isolated Lac- tobacillus can be used as potential probiotics,but the protection mechanism should be further in- vestigated.