猪干扰素调节因子1在细胞抗病毒天然免疫应答中的作用

Role of porcine interferon regulatory factor 1 in cellular anti-viral innate immune responses

从猪肺泡巨噬细胞中克隆了猪干扰素调节因子1(interferon regulatory factors 1,IRF1)基因,并构建了真核表达载体pCAGGS-HA-IRF1。将该质粒转染PK-15后通过Western-blot确定IRF1蛋白在细胞内能够高效表达。通过体外抗病毒试验发现,过表达IRF-1能够显著抑制H1N1流感病毒和猪水泡性口炎病毒的复制。进一步通过双荧光报告系统发现,IRF1显著促进polyI:C诱导的猪IFN-β、ISRE和NF-κB启动子的活性,说明IRF1可能通过增强细胞的天然免疫应答进而发挥抗病毒功能。

In order to study the anti-viral effects and immune-modulatory actions of IRF1 ,porcine IRF1 gene was cloned firstly from porcine pulmonary alveolar epithelial cells and inserted into the plasmid of pCAGGS （designed as pCAGGS-HA-IRF1）, pCAGGS-HA-IRF1 was transfected into PK-15 cells and subseaquently Western-blot corfirmed the highly effective expression of IRF1 protein. In vitro antiviral tests showed that over-expression of IRF-1 can significantly inhibit the replication of H1N1 influenza virus and swine vesicular stomatitis virus. Further studies by dual luciferase reporter system showed that IRF1 significantly promoted promoter activities of polyI ：C-induced porcine IFN-~, ISRE and NF-~：B, indicating that IRF1 may thus play the anti-viral role by enhancing cellular innate immune response. This study will be contributed to further explore the antiviral effects and immune-modulatory functions of porcine IRF1.