Hmgn3和Hoxa10对小鼠子宫基质细胞蜕膜化过程中Foxo1表达的调控

Regulation of Hmgn3 and Hoxa10 on Foxo1 expression in the decidualization process of uterine stromal cells in mice

利用小鼠子宫基质细胞体外诱导蜕膜化模型转染Hmgn3或Hoxa10过表达载体及siRNA片段,通过荧光定量PCR方法检测Hmgn3和Hoxa10对Foxo1表达的调控。结果显示:转染Hmgn3或Hoxa10过表达载体可促进Foxo1在子宫基质细胞蜕膜化过程中的表达,而转染Hmgn3或Hoxa10siRNA则可抑制Foxo1的表达。在小鼠子宫基质细胞中转染Hmgn3或Hoxa10siRNA后再添加孕酮,Foxo1的表达显著下降。同样,干扰Hmgn3或Hoxa10也可减低cAMP对Foxo1表达的调控。结果表明:Hmgn3和Hoxa10可通过Foxo1来影响小鼠子宫基质细胞的蜕膜化,孕酮和cAMP可通过Hmgn3和Hoxa10来调控Foxo1在子宫基质细胞中的表达。

Uterine stromal decidualization is very important for the maintenance of normal preg- nancy in mammals. The previous research has confirmed that Hmgn3 and Hoxal0 play an impor- tant role in the process of deeidualization, but little is known about the regulatory mechanism of Hmgn3 and Hoxal0 during decidualization. In this study,we transfected the uterine stromal cells with overexpression plasmids or siRNAs for Hmgn3 or Hoxal0, then added with estrogen and progesterone to induce decidualization in vitro and examined the regulation of Hmgn3 and Hoxal0 on the expression of Foxol by real-time PCR. The results showed that Foxol expression was ob- viously enhanced in the uterine stromal cells treated with estrogen and progesterone after trans- fection with Hmgn3 or Hoxal0 overexpression plasmid, but inhibited after transfection with Hmgn3 or Hoxal0 siRNA, respectively. After the stromal cells were transfected with Hmgn3 or Hoxal0 siRNA and then added progesterone, the expression of Foxol was significantly diminished. Like- wise,knockdown of Hmgn3 or Hoxal0 with specific siRNA could obviously attenuate the regulation of cAMP on the expression of Foxol. In conclusion, Hmgn3 and Hoxal0 may regulate the decidualization of mouse uterine stomal cells by affecting the expression of Foxol. Progesterone and cAMP regulate the ex- pression of Foxol in uterine stomal cells through Hmgn3 and Hoxal0.