不同剂量丹参注射液对冻融小鼠卵巢移植早期组织结构的影响

Effect of different doses of salvia miltiorrhiza injection on the structures during the early ovarian transplantation in mice

目的探讨不同剂量丹参注射液对冻融小鼠卵巢移植早期组织结构的影响。方法收集1日龄小鼠卵巢，慢速冻融后移植至F1代8～12周雄鼠的肾被膜下，分生理盐水组和低（0．4ml／kg，n=15）、中（4ml／kg，n=15）、高（40ml／kg，n=15）剂量丹参干预组（n=15），分别于移植后第2天、第7天和第14天回收12组卵巢移植物。观察冻融卵巢组织移植前后的组织形态学及超微结构改变，计数卵泡数量。结果光学显微镜、电子显微镜下观察显示，各组卵巢组织移植后7d卵泡细胞和颗粒细胞结构欠完整，移植后14d卵泡结构恢复正常。移植后14d高剂量丹参组（36．2％）各级卵泡数及卵泡存活率多于中、低剂量丹参组（24．2％、20．5）和生理盐水组（12．6％），差异显著（P〈0．05）。结论移植早期的缺血缺氧可对小鼠卵巢组织造成比冻融过程更严重的损伤；丹参能促进移植后冻融小鼠卵巢卵泡发育，以高剂量丹参作用最明显。

Objective To investigate the effect of different doses of salvia mihiorrhiza injection on the structures during the early ovarian transplantation in mice. Methods Ovaries from 1 day old mice were collected and cryoprescrved. The thawed ovaries were xenografted into kidney capsules of 8-12 weeks old adult male mice. The mice were divided into four groups： low doses（0.4ml/kg, n = 15） , middle doses （4ml/kg, n = 15） , high doses （40ml/kg, n = 15） salvia mihiorrhiza group and saline group （ n = 15）. The grafted ovaries were collected 2, 7, and 14 days after transplantation for evaluating. The morphology and ultrastructure of the tissue before and after freezing and thawing ovarian tissue transplantation were observed. Results The histological structure and uhrastructure showed that both the follicles and stromata were damaged on day 7 after transplantation. The follicular structure was recovered for 14 days. After transplantation the number of follicles and survival rates in graft were higher in high dose salvia miltiorrhiza group （36. 2% ） than those in middle dose （24.2%） , low dose （20.5%） ,and saline group（ 12.6% ） on day 14 （ P 〈 O. 05 ）. Conclusion Ischemia and hypoxia of mouse ovarian tissues during early transplantation caused by far mouse serious injury than freezing and thawing processes. Salvia mihiorrhiza particularly the high dose can promoted the follicular development of the frozen-thawed mouse ovarian after transplanation.