消痰散结方对裸鼠人胃癌细胞SGC-7901皮下移植瘤RUNX3、NF-κB p65蛋白表达的影响

Effect of “Xiaotan Sanjie Decoction”on RUNX3 and NF-κB p65 protein expression in Human gastric cancer cell SGC-7901-bearing nude mice

目的观察消痰散结方对裸鼠人胃癌细胞SGC-7901皮下移植瘤Runt相关转录因子3(RUNX3)、核转录因子κB p65(NF-κB p65)蛋白表达的影响,探讨消痰散结方的抗胃癌作用及机制。方法建立裸鼠人胃癌细胞SGC-7901皮下移植瘤模型,随机分为中药组(消痰散结方)、化疗组(卡培他滨)及对照组(0.9%Na Cl溶液),每组10只,各组给予相应干预共32天。观察各组裸鼠体质量、瘤体积、瘤重,并采用Western blotting法检测各组裸鼠皮下移植瘤组织中RUNX3、NF-κB p65蛋白表达水平。结果中药组及化疗组的瘤体积及瘤重值均低于对照组(P<0.05);与中药组比较,化疗组的瘤体积及瘤重值均下降,差异有统计学意义(P<0.05)。中药组和化疗组的抑瘤率分别为44.91%和83.23%。中药组和对照组体质量差异无统计学意义(P>0.05);化疗组体质量低于对照组及中药组(P<0.05)。中药组和化疗组的肿瘤组织RUNX3蛋白表达水平高于对照组、NF-κB p65蛋白表达水平低于对照组(P<0.05)。结论消痰散结方具有较好的抗胃癌疗效,虽然在抑瘤率方面不及卡培他滨,但没有引起体质量下降的副作用;上调RUNX3蛋白并下调NF-κB p65蛋白表达水平是其可能的作用机制之一。

Objective To observe the effect of＂Xiaotan Sanjie Decoction＂on the RUNX3 and NF-κB p65 protein expression in Human gastric cancer cell SGC-7901-bearing nude mice,and discuss the anti-gastric cancer effect and mechanism of ＂Xiaotan Sanjie Decoction＂. Methods Human gastric cancer cell SGC-7901-bearing nude mice models were made. The mice were divided into TCM group（ ＂Xiaotan Sanjie Decoction ＂）,chemotherapy group（ capecitabine） and control group（ 0. 9% Na Cl saline） randomly,10 in each group,with 32 d intervention respectively in each group. The tumor volume,tumor weight,body weight,RUNX3 and NF-κB p65 protein expression in tumor were observed the the method of Western blotting. Results The tumor volume and weight of TCM group and chemotherapy group were lower than those of control group（ P 〈 0. 05）; compared with TCM group,the tumor volume and weight in chemotherapy group decreased,and the difference was statistically（ P 〈 0. 05）; The anti-tumor rate of TCM group and chemotherapy group were 44. 91% and 83. 23% respectively.There was no statistical significance in weight between TCM group and control group（ P 〉 0. 05）. The body weight in chemotherapy group decreased compared with that in control group and TCM group（ P 〈 0. 05）. The RUNX3 protein expression in TCM group and chemotherapy group was higher than that in control group,while the NF-κB p65 expression was lower than that of control group（ P 〈 0. 05）. Conclusion ＂Xiaotan Sanjie Decoction＂has the good anti-gastric cancer effect.Although the anti-cancer rate of＂Xiaotan Sanjie Decoction＂was not as good as capecitabine,it had not bodyweight decreasing side effect. Increasing the RUNX3 expression and decreasing the NF-κB P65 protein expression in tumor tissues may account for the anti-gastric cancer mechanism of＂Xiaotan Sanjie Decoction＂.