川崎病患儿淋巴细胞亚群及免疫球蛋白的变化

Changes in lymphocyte subsets and immunoglobulin in children with Kawasaki disease

目的探讨检测川崎病(KD)患儿淋巴细胞亚群及免疫球蛋白的临床价值。方法入选388例KD患儿,160例感染发热性疾病患儿,85例健康体检的正常对照儿童;采用流式细胞仪检测T淋巴细胞亚群、自然杀伤细胞(NK细胞)和B淋巴细胞绝对值和百分比,速率散射比浊法检测血清Ig G,Ig M,Ig A和补体C3、C4水平。结果与感染发热性疾病患儿及正常对照儿童相比,KD患儿B细胞、补体C3和CD4/CD8比值显著升高,而CD8+T细胞和NK细胞百分比下降,CD3+T细胞的绝对值升高,差异均有统计学意义(P均<0.05)。结论淋巴细胞亚群和补体可作为鉴别KD和了解KD发病机制的实验室指标。

Objective To explore the clinical value of the analysis of lymphocyte subsets and immunoglobulin in children with Kawasaki disease （KD）. Methods Three hundred and eighty-eight children with KD, 160 children with infectious febrile disease and 85 normal children for well child visit were enrolled. The absolute value and percentage of T lymphocyte subsets, natural killer cells （NK cells） and B lymphocytes were detected by flow cytometry. The serum levels of IgG, IgM, IgA, and complement C3, C4 were assessed by velocity scatter turbidimetry. Results Compared with infectious febrile children and normal control children, the levels orB cells, complements C3 and ratio of CD4/CD8 ceils were significantly increased, the percentage of CD8＋T cells and NK cells significantly decreased, and the absolute values of CD3＋T cells significantly increased in children with KD （all P 〈 0.05）. Conclusions Lymphocytes subsets and complements might be used as laboratory index to identify KD and understand the pathogenesis of KD.