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体外成球实验筛选三阴乳腺癌干性相关microRNA实验研究 被引量：5

Spheroid Study in Vitro on Screening the microRNA Associated with Breast Cancer Stem Cells of Triple Negative Breast Cancer
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摘要 目的筛选三阴乳腺癌(triple negative breast cancer,TNBC)干性相关microRNA(miRNA),为TNBC不良预后预测提供干性靶标。方法贴壁培养人乳腺癌高转移潜能细胞系MDA-MB-231进行体外成球实验,分别抽提Spheroid和Attachment培养细胞总RNA,利用miRNA芯片比较两种培养细胞表达miRNA的差异情况。结果 Spheroid及Attachment细胞表达差异的miRNA共有157个,其中10个miRNA表达差异最明显,且差异倍数达4倍以上。结论乳腺癌干细胞中差异表达的miRNA可能与TNBC耐药相关,可作为诊断乳腺癌和评判预后的重要靶标,并为抵抗乳腺癌耐药提供可能靶点。 Objective To screen and identify the miRNA associated with breast cancer stem cells by the miRNA ar- ray technique, and predict miRNA associated with drug resistance of TNBC, in order to provide target gene for poor prognosis prediction of TNBC. Methods Total RNA was respectively extracted from Spheroid and attachment culture MDA-MB-231, and the experiment was conducted in vitro. Results Spheroid and attachment culture cell expressed a total of 157 miRNA with differences, including 10 miRNAs with the most significant differences at 4 times or over. Conclusion Differentially expressed miRNAs in stem cells of breast cancer may be related to drug resistance of TNBC. It can be used as an important cri- terion in diagnosis of breast cancer and provide a target point for drug resistance of TNBC.
作者 谢晚晴 李佳 郭东辉 蒋宁 孙丽 张云峰 郑洪新
机构地区 辽宁中医药大学
出处 《临床误诊误治》 2016年第1期113-116,共4页 Clinical Misdiagnosis & Mistherapy
基金 沈阳市科技计划项目(F13303400)
关键词 乳腺肿瘤 微RNAS 肿瘤干细胞 抗药性 肿瘤 Breast neoplasm MicroRNAs Neoplastic stem cell Drug resistance, neoplasm
分类号 R737.9 [医药卫生—肿瘤]
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参考文献14

  • 1杜成,刘兆喆,马东初,射晓冬.MTDH 基因下调抑制人乳腺癌 MDA-MB-453细胞增殖同黏附和迁移的研究[J].中国肿瘤临床,2012,29(8):425-428. 被引量：7
  • 2Metzger-Filho O, Tutt A, de Azambuja E, et al. Dissec- ting the heterogeneity of triple-negative breast cancer[ J ]. J Clin Oncol, 2012,30(15) :1879-1887.
  • 3Foulkes W D, Smith I E, Reis-Filho J S, et al. Triple- negative breast cancer [ J]. N Engl J Med, 2010, 365 ( 20 ) : 1938-1948.
  • 4AI-Hajj M, Wicha M S, Benito-Hernandez A, et al. Pro- spective identification of tumorigenic breast cancer cells [J]. Proc Natl Acad Sci USA, 2003, 100 (7) : 3983- 3988.
  • 5So J Y, Lin J J, Wahler J, et al. A synthetic triterpenoid CDDO-Im inhibits tumorspherc formation by regulating stem cellsignaling pathways in trlple-negative breast canc- er[J]. PLoS One, 2014,9(9) :e107616.
  • 6Le X F, Merchant O, Bast R C, et al. The Roles of Mi- croRNAs in the Cancer Invasion-Metastasis Cascade [ J ]. Cancer Microenviron, 2010,3 ( 1 ) : 137-147.
  • 7Buffa F M, Camps C, Winchester L, et al. microRNA- associated progression pathways and potential therapeutic targets identified byintegrated mRNA and microRNA ex- pression profiling in breast cancer[ J]. Cance Res, 2011, 71 (17) :5635-5645.
  • 8Rothe F, Ignatiadis M, Chaboteaux C, et al. Global mi- croRNA expression profiling identifies miR-210 associated with tumor proliferation, invasion and poor clinical out- come in breast cancer[J]. PLoS One, 2011,6 (6): e20980.
  • 9刘志勇,孙保存.肿瘤干细胞与肿瘤血管异质性[J].中国肿瘤临床,2014,41(2):134-137. 被引量：5
  • 10A1-Hajj M, Wicha M S, Benito-Hernandez A, et al. Pro- spective identication of tumorigenic breast cancer cells [J]. Proc Natl Acad Sci USA, 2003, 100 (7):3983- 3988.

二级参考文献70

  • 1Jemal A,Bray F,Center MM,et al.Global cancer statistics[J].CA Cancer J Clin.2011,61(2):69-90.
  • 2Su ZZ,Chen Y,Kang DC,et al.Customized rapid subtraction hybridization (RaSH) gene microarrays identify overlapping expression changes in human fetal astrocytes resulting from human immunodefi ciency virus-1 infection or tumor necrosis factor-alpha treatment[J].Gene,2003,306:67-78.
  • 3Brown,DM.,Ruoslahti,E.Metadherin,a cell surface protein in breast tumors that mediates lung metastasis[J].Cancer Cell,2004,5(4):365-374.
  • 4Li J,Zhang N,Song LB,et al.Astrocyteelevated gene-1 is a novel prognostic marker for breast cancer progression and overall patient survival[J].Clin Cancer Res,2008,14(11):3319-3326.
  • 5Yoo BK,Rachel G,Nicollaq V,et al.Identification of genes conferring resistance to 5-fluorouracil[J].Proc Natl Acad Sci U S A,2009,106(31):12938-12943.
  • 6Emdad L,Lee SG,Su ZZ,et al.Astrocyte elevated gene-1 (AEG-1) functions as an oncogene and regulates angiogenesis[J].Proc Natl Acad Sci USA,2009,106(50):21300-21305.
  • 7Yoo BK,Emdad L,Lee SG,et al.Astrocyte elevated gene-1 (AEG-1):A multifunctional regulator of normal and abnormal physiology[J].Pharmacol Ther,2011,130(1):1-8.
  • 8Liu HY,Song XR,Liu CX,et al.Knockdown of astrocyte elevated gene-1 inhibits proliferation and enhancing chemo-sensitivity to cisplatin or doxorubicin in neuroblastoma cells[J].J Exp Clin Cancer Res,2009,28(1):19-27.
  • 9Douglas H,Robert AW.Hallmarks of cancer:the next generation[J].Cell,2011,144(5):646-674.
  • 10Li J,Yang L,Song L,et al.Astrocyte elevated gene-1 is a proliferation promoter in breast cancer via suppressing transcriptional factor FOXO1[J].Oncogene,2009,28(36):3188-3196.

共引文献10

同被引文献40

  • 1Yu C,Wang J. A Physical Mechanism and Global Quan-tification of Breast Cancer[J]. PLo S One,2016,11 ( 7) :e0157422.
  • 2Parsa Y,Mirmalek S A,Kani F E,et al. A Review ofthe Clinical Implications of Breast Cancer Biology[J].Electron Physician,2016,8( 5) : 2416-2424.
  • 3Kuznetsova E S,Zinovieva O L,Oparina N Y,et al. Ab-normal expression of genes that regulate retinoid metabo-lism and signaling in non-small-cell lung cancer[J]. MolBiol ( Mosk) ,2016,50( 2) : 255-265.
  • 4Hu L,Chen H Y,Han T,et al. Downregulation ofDHRS9 expression in colorectal cancer tissues and itsprognostic significance[J]. Tumour Biol,2016,37 ( 1 ) :837-845.
  • 5Kropotova E S,Zinovieva O L,Zyryanova A F,et al.Altered expression of multiple genes involved in retinoicacid biosynthesis in human colorectal cancer[J]. PatholOncol Res,2014,20( 3) : 707-717.
  • 6Wang C,Kane M A,Napoli J L. Multiple retinol andretinal dehydrogenases catalyze all-trans-retinoic acid bio-synthesis in astrocytes[J]. J Biol Chem,2011,286 ( 8 ) :6542-6553.
  • 7Jones R J,Dickerson S,Bhende P M,et al. Epstein-Barr virus lytic infection induces retinoic acid-responsivegenes through induction of a retinol-metabolizing enzyme,DHRS9[J]. J Biol Chem,2007,282( 11) : 8317-8324.
  • 8Al-Hajj M. Cancer stem cells and oncology therapeutics[J]. Curr Opin Oncol,2007,19( 1) : 61-64.
  • 9Al-Hajj M,Wicha M S,Benito-Hernandez A,et al. Pro-spective identification of tumorigenic breast cancer cells[J].Proc Natl Acad Sci U S A,2003,100( 7) : 3983-3988.
  • 10Bozorgi A,Khazaei M,Khazaei M R. New Findings onBreast Cancer Stem Cells: A Review[J]. J Breast Canc-er,2015,18( 4) : 303-312.

引证文献5

二级引证文献12

临床误诊误治
2016年 第1期

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