摘要
目的探讨养心复脉饮对大鼠肝微粒体细胞色素P450(CYP)酶1A2、2E1及3A4各亚型的影响。方法 SD大鼠24只,随机分为4组:正常对照组和养心复脉饮小、中、大剂量组,每组6只,分别灌胃给予0.9%氯化钠注射液5.00 m L·kg-1和养心复脉饮2.50,5.00 10.00 m L·kg-1,连续14 d;采用差速离心法分离肝微粒体,高效液相色谱(HPLC)法分别测定大鼠肝微粒体中CYP1A2、2E1及3A4探针药物的代谢率。结果在体外肝微粒体孵育体系中,养心复脉饮小、中剂量组CYP1A2、CYP2E1和CYP3A4活性与正常对照组差异无统计学意义(P>0.05);大剂量组CYP1A2、CYP2E1和CYP3A4活性分别为(28.43±4.49)%,(42.23±1.44)%,(11.20±0.98)%,均比正常对照组[分别为(15.51±4.26)%,(31.54±3.37)%,(5.66±0.65)%]升高(均P<0.05)。结论养心复脉饮对大鼠肝微粒体CYP1A2、2E1以及3A4有明显的诱导作用。
Objective To investigate the influence of Yangxin Fumai potion on liver microsomal CYP1A2,2E1 and3A4 in rats. Methods Twenty-four SD rats were divided into 4 groups at random（ n = 6） ： the experimental groups were given with Yangxin Fumai potion at 2.50,5.00 and 10.00 m L·kg^-1,respectively,by gastric gavage for 14 days,while the normal control group was given with 0.9% sodium chloride injection at 5.00 m L·kg^-1. Upon differential centrifugation of liver microsomals,the metabolic rates of CYP 1A2,2E1 and 3A4 probe drugs were detected by HPLC. Results No significant difference was presented in the Yangxin Fumai potion at low and medium doses,which compared with those in the normal control group（ P 0.05） in vitro.The activities of CYP1A2,CYP2E1 and CYP3A4 in the high dosage of Yangxin Fumai potion group were（ 28.43±4.49） %,（ 42. 23 ± 1.44） %,（ 11.20± 0. 98） %,respectively,which were higher than those in the normal control group（ 15. 51 ±4.26） %,（ 31.54±3.37） %,（ 5.66± 0. 65） %,respectively（ P 〈0. 05）. Conclusion Yangxin Fumai potion can activate liver microsomal CYP 1A2,2E1 and 3A4 of rats.
出处
《医药导报》
CAS
2016年第1期47-49,共3页
Herald of Medicine
基金
2012年浙江省中医药重点研究计划(2012ZB167)
