一个新的玉米黄绿叶突变体ygl-m的初步研究

The initial research of a new yellow-green leaf mutant ygl-m in maize

在田间选育系谱过程中发现了一份黄绿叶突变体ygl-m,该突变体叶片在苗期自发地表现黄绿色,待植株长到6周大左右植株叶片开始恢复绿色,最后整个植株叶片都恢复正常的绿色。苗期ygl-m与野生型植株B73相比,叶片总叶绿素、叶绿素a、叶绿素b含量均显著下降,叶绿素a/b比值显著升高;苗期叶片叶绿体中基粒类囊体片层较少,排列不规则,结构松散。遗传分析表明,突变体ygl-m的黄绿叶表型由隐性单基因控制。本研究将为开展ygl-m基因的分子标记定位和进一步探讨其利用潜力奠定基础。

A yellow-green leaf mutant ygl-m was identified in the process of pedigree selection in field,which spontaneously showed yellow-green leaf phenotype at seedling stage. The plants began to restore green in almost 6weeks later,and then all leaves of the plants turned green. Compared to wild type line B73,the contents of total chlorophyll,chlorophyll a,chlorophyll b in ygl-m decreased significantly,while the ratio of chlorophyll a / b increased significantly. There are only of few chloroplast grana thylakoid lamellar with irregular arrangement and loose structure in ygl-m at seedling stage. Genetic analysis showed that a single recessive gene controlled the yellowgreen leaf phenotype of ygl-m. This study may lay foundations to molecular map of ygl-m and further explore its application of potential.