聚合人脐带血红蛋白对大鼠心肌组织影响的初步探讨

Preliminary study on the effect of polymerized human cord hemoglobin on rat myocardial tissue

目的探讨聚合人脐带血红蛋白(PolyCHb)对动物心肌组织的影响。方法取18只健康雄性SD大鼠随机均分为假手术(Sham)组:大鼠麻醉后仅行股动、静脉插管,颈静脉及右侧颈总动脉插管;NaCl组及PolyCHb组:除同Sham组一样麻醉、插管后,分别输注生理盐水或PolyCHb(800 mg/kg);连续监测基础值、输注后0、10、20、30、60 min 3组大鼠的平均动脉压(MAP)、心率(HR)、左心室收缩末期压力(pes)、左心室舒张末期压力(ped)、室内压最大上升速率(dp/dt max)、室内压最大下降速率(dp/dt min)、等容舒张时间常数(Tau)。另取18只大鼠同样分作3组,麻醉、插管后按分组做相应处理,取输后1、3、6 h血样于EDTA抗凝管中,离心取血浆,测定肌酸激酶同工酶(CK-MB)及肌钙蛋白Ⅰ(cTnⅠ)含量,6 h时处死大鼠,取心脏做病理学检测。结果 PolyCHb组、 NaCl组与sham组比较:1)术(输)后0—60 min MAP(mmHg)及pes(mmHg)持续升高,至输注后60 min时,分别为128.50±7.26 vs 115.67±4.72 vs 106.5±14.84(P<0.05)与148.30±18.16 vs 122.83±13.57 vs 122.07±19.00(P<0.05);术(输)后HR(次/min):0 min时分别为302.83±20.76vs 371.00±43.36 vs 387.50±47.27 (P<0.05), 60 min时分别为391.50±34.26 vs 416.83±33.47 vs 431.50±33.76 (P>0.05); ped(mmHg):60 min时分别为11.34±11.58 vs 6.83±4.79 vs 8.14±4.25(P>0.05);2)dp/dt max(mmHg/s)与dp/dt min(mmHg/s)术(输)注0 min分别为6 530.50±418.63 vs 8 341.50±1 095.00 vs 8 242.17±1 579.38与5 352.83±449.02 vs 7 687.00±1 111.87 vs 7 799.50±2 246.39(P<0.05),至术(输)后60 min分别为8 139.00±758.26 vs 10 083.33±1 256.59 vs 10 032.17±1 779.30与7 457.33±915.62 vs 9 192.00±892.90 vs 9 069.50±2 104.49(P<0.05);3)Tau术(输)后0 min分别为17.01±3.72 vs 12.69±3.32 vs 12.57±2.07(P<0.05);4)CK-MB(pg/mL)术(输)后1、3 h术(输)后1、3 h时分别为63.41±13.15 vs 49.67±20.10 vs 38.20±6.02与101.88±28.08 vs 37.80±18.21 vs 37.94±10.71(P<0.05);5)cTnI(pg/mL)术(输)后3 h分别为14.61±7.03 vs 5.32±2.96 vs 5.72±2.90(P<0.05)。PolyCHb组大鼠术(输)后6 h病理检查未见心肌损伤。结论输注PolyCHb可影响心肌功能,酶学水平升高,但不会造成病理损伤。

Objective To investigate the effects of polymerized human cord hemoglobin(PolyCHb)on myocardial tissue in animals.Methods Eighteen healthy male Sprague-Dawley rats were randomly divided into sham operation group,NaCl group and PolyCHb group.Rats in sham operation group were anesthetized with femoral artery,venous cannula,jugular vein and right common carotid artery cannula;rats in NaCl group and PolyCHb group were infused with saline or PolyCHb(800 mg/kg)separately besides anesthesia and intubation conducted in the sham group;the mean arterial pressure was continuously monitored at the baseline,0,10,20,30,and 60 min after infusion.Mean arterial pressure(MAP),heart rate(HR),left ventricular end-systolic pressure(pes),left ventricular end-diastolic pressure(ped),maximum rate of increase in indoor pressure(dp/dt max),maximum rate of decline in indoor pressure(dp/dt min),isovolumic relaxation time constant(Tau)of the rats in three groups were tested.Another 18 rats were also divided into 3 groups.After anesthesia and intubation,the rats were treated according to the group.The blood samples were taken in the EDTA anticoagulation tube at 1,3,and 6 h after infusion,and the plasma was centrifugated to determine the creatine kinase isoenzyme(CK-MB)and troponin I(cTnI)levels.The rats were sacrificed at 6 h,and the heart was taken for pathological examination.Results The related parameters in PolyCHb group,the NaCl group and the sham group were compared as follows:1)MAP(mmHg)and pes(mmHg)continued to increase during 0—60 min after surgery(or infusion),and reached to 128.50±7.26 vs.115.67±4.72 vs.106.5±14.84(P<0.05)and 148.30±18.16 vs.122.83±13.57 vs.122.07±19.00(P<0.05),respectively,at 60 min;HR(number of times/min)at 0 and 60 min after surgery(or infusion)was 302.83±20.76 vs.371.00±43.36 vs.387.50±47.27(P<0.05)and 391.50±34.26 vs.416.83±33.47 vs.431.50±33.76(P>0.05);ped(mmHg)at 60 min was 11.34±11.58 vs.6.83±.4.79 vs.8.14±4.25(P>0.05);2)dp/dt max(mmHg/s)and dp/dt min(mmHg/s)at 0 min were 6350.50±418.63 vs.8341.50±1095.00 vs.8242.17±1579.38 and 5352.83±449.02 vs.7687.00±1111.87 vs.7799.50±2246.39(P<0.05);at 60 min after surgery(or infusion)were 8 139.00±758.26 vs.10 083.33±1256.59 vs.10032.17±1779.30 and 7 457.33±915.62 vs 9192.00±892.90 vs.9069.50±2104.49(P<0.05);3)Tau at 0 min was 17.01±3.72 vs.12.69±3.32 vs.12.57±2.07(P<0.05);4)CK-MB(pg/mL)at 1 and 3 h after surgery(or infusion)was 63.41±13.15 vs.49.67±20.10 vs.38.20±6.02 and 101.88±28.08 vs.37.80±18.21 vs.37.94±10.71,respectively(P<0.05);5)cTnI(pg/mL)at 3 h after surgery(or infusion)was 14.61±7.03 vs.5.32±2.96 vs.5.72±2.90(P<0.05).No myocardial injury at 6 h after surgery(or infusion)was observed in the PolyCHb group.Conclusion Infusion of PolyCHb can affect myocardial function and increase the enzymology level,but it will not cause pathological damage.