不同程度乳糜血制备洗涤红细胞的保存期中溶血率的初步分析

Preliminary analysis of hemolysis rate of MAP suspend washed red blood cells prepared from fat blood with different chyle degrees

目的探讨不同程度乳糜血制备的红细胞保存液(MAP)混悬洗涤红细胞在保存期内的溶血率。方法将MAP混悬洗涤红细胞分对照、轻、中、重度乳糜血在2—6℃保存后测定其储存期末溶血率,并分别在储存期的d2、d7、d14、d21、d28、d30、d35取样检测红细胞的溶血率。结果 MAP混悬洗涤红细胞对照组、轻、中、重度乳糜组的储存期末溶血率分别为:(0. 156±0. 027)%、(0. 174±0. 049)%、(0. 271±0. 066)%、(0. 463±0. 127)%。MAP混悬洗涤红细胞乳糜程度不同,不同储存期红细胞溶血率均差异显著(P<0. 05)。结论在保存期内,使用轻中度乳糜血制备的MAP混悬洗涤红细胞质量控制指标符合国家标准要求。

Objective To investigate the hemolysis rates of washed red blood cells with MAP maintenance solution prepared from fat blood with different chyle degrees.Methods The MAP suspend washed red blood cells were divided into three groups according to the chyle severity(mild,moderate and severe)and stored at 2—6℃.The hemolysis rate of them on day 2,7,14,21,28,30 and 35,respectively,during storage and at the end of storage were measured.Results The end-storage hemolysis rates of MAP suspend washed red blood cells in control group,mild,moderate and severe chyle group were(0.156±0.027)%,(0.174±0.049)%,(0.271±0.066)%and(0.463±0.127)%,respectively.The hemolysis rate had significant difference with chyle degrees and storage periods.Conclusion During the storage period,the quality of MAP suspend washed red blood cells prepared from mild and moderate chylemia meets the requirements of national standards.