加系SPF大白猪和长白猪群体遗传学分析

Analysis of the pedigreed population genetics of SPF Yorkshire and Landrace pigs imported from Canada

目的研究中国农业科学院哈尔滨兽医研究所从加拿大引进的SPF大白猪和长白猪的遗传学背景。方法实验采用19对微卫星引物对该群体进行群体遗传学分析。结果 19个位点在大白猪群中检测到84个等位基因,长白猪群中检测到89个等位基因。大白猪的平均多态信息含量和平均杂合度分别为0.5271和0.5877;长白猪的平均多态信息含量和平均杂合度分别为0.5652和0.6066。由于S0155、S0143、S0178、Sw857和Sw936位点内等位基因大小和含量差异显著(P<0.01)可作为大白猪和长白猪品种鉴定的候选位点。F-统计和迁移率分析结果表明,群体内的分化较小,遗传结构稳定。结论引进加系SPF纯种大白猪和长白猪的遗传结构与国内部分纯种大白猪和长白猪相比更为稳定,可作为实验动物模型应用于动物医学和科学研究。

Objective To understand the genetic background of the specific pathogen-free Yorkshire and Landrace pigs,imported from Canada by Harbin Veterinary Research Institute,Chinese Academy of Agricultural Sciences. Methods The population genetics were investigated by using 19 pairs of microsatellite primers. Results In the Yorkshire pigs and Landrace pigs,84 and 89 alleles,respectively,were detected at 19 microsatellites loci. The average polymorphic information content and mean heterozygosity in the Yorkshire pigs were 0. 5271 and 0. 5877,and in the Landrace population were0. 5652 and 0. 6066,respectively. Because of the significant（ P〈0. 01） differences of alleles in different loci such as S0155,S0143,S0178,Sw857 and Sw936,it made them possible to be used to identify Yorkshire and Landrace pigs＇ breed.F-statistics showed that the differentiation within the population was small and genetic structure was stable. Conclusions Compare with the domestic pedigree large White and Landrace pigs,the SPF pedigree pigs imported from Canada are more stable in genetic structure,and can be used as laboratory animal models in animal science research.