甘蓝型油菜低温诱导下的bZIP转录因子挖掘及聚类分析

Mining and Cluster Analysis of Rapeseed bZIP Transcription Factors Induced by Low Temperature

植物响应低温胁迫的过程是一个复杂的网络过程,涉及众多转录因子的调控。本文通过对低温胁迫下的甘蓝型油菜转录组数据进行生物信息学分析,获得108个序列编码bZIP转录因子。经过序列比对、染色体定位和聚类分析,发现这些转录因子定位在19条染色体上,在抗寒过程中表现动态变化;同时发现这些转录因子在A亚族、C亚族、I亚族、G亚族、S亚族中聚集最多,B亚族和D亚族中很少聚集;取4个在所有胁迫阶段皆表现差异的基因进行定量分析,发现其调控变化与测序分析的变化趋势相同,不仅证明了测序数据的可靠性,而且证明甘蓝型油菜bZIP转录因子对低温胁迫的响应是一个动态的调控过程。

Plant response to low temperature stress is a complex network process,involving numerous of transcription factors regulation. In this paper,we had found 108 full length cDNA sequences encoding b ZIP factors in rapeseed from a transcriptome database bioinformatics analysis under low temperature stress. Through sequence compare,chromosome distribution and cluster analysis,these sequences could be differently distributed on the 19 chromosomes,performing dynamic change in cold resistance process. We also found these transcription factors in A subtribe,C subtribe,I subtribe,G subtribe,S subtribe gathered the most,the subtribe B and D subtribe rarely gathered.Quantitative analysis of the four genes differences performance in all stress stages,we found that the genes regulation had the same trend with the sequencing analysis,not only proved the reliability of the sequencing data,but also proved the dynamic regulation of rapeseed b ZIP transcription factors in response to low temperature stress.