普通烟草ARF基因家族序列的鉴定与表达分析

Identification and Expression Analysis of the ARF Gene Family in Nicotiana Tabacum

ARF(AUXIN RESPONSE FACTOR)基因含有一个B3功能域和具有转录激活或抑制活性的中心功能域,在植物发育过程中起到非常重要的作用。本研究采用生物信息学方法,根据拟南芥ARF基因序列鉴定了普通烟草基因组中的ARF基因,并对家族成员进行了序列特征、系统发生、亚细胞定位和表达模式分析。目前在普通烟草基因组中共得到50个ARF基因成员,其基因结构相对复杂,一般含有10个外显子。亚细胞定位结果表明,少数ARF蛋白定位到线粒体或叶绿体,大多数未检测到定位信号。转录组数据分析表明,ARF基因具有不同的组织表达模式,部分基因表现出组织特异性。这些研究结果为普通烟草ARF基因家族功能的深入研究奠定了基础。

AUXIN RESPONSE FACTOR（ ARF） gene contains a B3 domain and a transcriptional activation or repression domain,which plays very important role in plant development. Base on bioinformatic method,the ARF genes of Nicotiana tabacum were screened with the ARF gene sequences of Arabidopsis. The sequence structure,phylogenetic relationship,subcellular localization and expression pattern of the identified gene sequences were analyzed. A total of 50 ARF genes were identified from tobacco with complex genetic structures contained 10 exons generally. The analysis of subcellular localization showed that a few of ARF proteins harbored mitochondrial or chloroplast localization sequences. The majority of ARF proteins were not found located signals. The analysis of expression pattern showed that the ARF genes had different expression patterns and tissue specificity. These results provided abundant information for further functional analysis of the ARF genes family.