哮喘小鼠CD4^+CD25^+调节性T细胞及相关细胞因子的变化研究

The expression of CD4^+CD25^+ regulatory T cells,IL-10,TGF-β and IL-17 in asthmatic mice

目的探讨哮喘小鼠CD4+CD25+调节性T细胞(regulatory T cells,Treg)及IL-10、TGF-β、IL-17水平的变化。方法 30只雄性BALB/c小鼠被随机分为三组:正常对照组、哮喘组及地塞米松组各10只;利用鸡卵白蛋白腹腔注射、雾化吸入制备哮喘模型;取小鼠左肺组织作病理切片观察炎症改变;通过流式细胞仪检测各组小鼠脾脏单个核细胞CD4+CD25+Treg细胞占CD4+T细胞的百分比;采用酶联免疫吸附试验检测各组小鼠血清中细胞因子IL-1、TGF-β、IL-17表达水平。结果哮喘组小鼠的脾单个核细胞CD4+CD25+Treg细胞百分比以及IL-10、TGF-β的表达水平较正常对照组降低(P<0.05),哮喘组IL-17的表达水平较正常对照组增高(P<0.05),地塞米松组与正常对照组比较,差异无统计学意义(P>0.05)。结论哮喘小鼠体内CD4+CD25+Treg数量的减少以及功能的下降、细胞因子IL-17表达的增加均参与了哮喘的发病过程。

Objective To discuss the expression of CD4＋ CD25＋ regulatory T cells （Treg）, IL-10, TGF-β and IL- l7 in asthmatic mice. Methods 30 male BALB/c mice were randomly assigned to the control group,the asthma group and the dexamethasone group. Tissues of left lung were obtained from mice to observe pathological changes. Flow cytom- etry was used to detect the percentage of CD4＋ CD25＋ Treg in CD4＋ T cells. The levels of serum IL-10,TGF-βand IL-17 were detected by ELISA. Results The percentage of CD4＋ CD25＋ Treg, IL-10 and TGF-βin asthma group decreased sig- nificantly （P〈0.05）. The expression of IL-17 in asthma group increased significantly（P〈0.05）. The difference was not significant between the control group and the dexamethasone group （P〉0.05）. Conclusion The number of CD4＋ CD25＋ Treg in asthmatic mice reduces, the function declines, and the increase of expression of IL-17 involves in the pathogenesis of asthma.