桑皮素通过抑制STAT3磷酸化下调卵巢癌SKOV3细胞Cyclin D1表达的实验研究

Morusin down-regulates Cyclin D1 expression by inactivation of STAT 3 in ovarian cancer SKOV3 cells

目的探讨桑皮素对卵巢癌SKOV3细胞的抑制作用和对Cyclin D1表达的调节作用及潜在的分子机制。方法使用不同浓度的桑皮素(0μM、10μM和50μM)对卵巢癌SKOV3细胞进行干预。在不同时间点(0h、24h和48h)使用MTT法对SKOV3细胞活性进行测定。在干预48h后,使用qPCR对在不同浓度桑皮素干预的SKOV3细胞Cyclin D1mRNA的表达水平进行测定。使用western blot对在不同浓度桑皮素的SKOV3细胞Cyclin D1蛋白表达水平和STAT3磷酸化水平进行测定。结果使用不同浓度桑皮素对卵巢癌SKOV3细胞进行干预后,细胞活性呈时间依赖性和浓度依赖性降低,差异均存在显著统计学意义(P均<0.05)。桑皮素干预48h后,卵巢癌SKOV3细胞Cyclin D1mRNA和蛋白的表达水平以及STAT3磷酸化水平水平呈浓度依赖性的下降,差异均存在显著统计学意义(P均<0.05)。结论本实验表明桑皮素可以通过抑制STAT3磷酸化显著抑制卵巢癌SKOV3细胞的增殖并下调Cyclin D1的表达。

Objective To explore the anti-tumor property of morusin in ovarian cancer SKOV3 cells and its under- lying mechanism. Methods SKOV3 cells were treated with three different concentrations （0μM, 10μM and 50μM） of morusin. At different time points （Oh, 24h and 48h）, MTT assay was used to detect the cell viabilities. QPCR was per- formed to measure the mRNA expression of Cyclin D1. The protein expression of Cyclin D1 and phosphorylation of STAT3 were analyzed by Western blot. Results The cell viabilities of ovarian cancer SKOV3 cells were concentration-de- pendently and time-dependently inhibited by morusin. The mRNA and protein expression of Cyclin D1 and the phospho- rylation of STAT3 were all concentration-dependently inhibited by morusin in ovarian cancer SKOV3 cells, after 48 hours interventions. Conclusions Morusin could suppress the proliferation of ovarian cancer SKOV3 cells and Cyclin D1 ex- pression probably through inactivation of STAT3.