摘要
目的观察不同剂量亚砷酸钠（NaAsO2）对PCI2细胞（具有神经元特性的大鼠嗜铬瘤细胞）色素上皮衍生因子（PEDF）和凋亡相关因子mRNA表达的影响。方法选择PC12细胞作为研究对象，给予不同剂量的NaAsO2[0（对照组）、2、5、10μmol／L]染毒24h，使用Real-time荧光定量PCR检测不同剂量NaAsO2染毒后PC12细胞的PEDF及其凋亡相关因子Bax和Bcl-2mRNA的表达水平。结果PEDFmRNA表达组间比较差异有统计学意义（F=102．28，P〈0．05），其中2、5、10μmol／LNaAsO2染毒组的PEDFmRNA表达（0．70±0．07、0．33±0．04、0．23±0．10）均低于对照组（1．15±0．11，P〈0．05）。BaxmRNA表达组间比较差异无统计学意义（0、2、5、10μmoVL组分另0为0．95±0．12、0．80±0．11、0．88±0．11、1．01±0．11，F=2．01，P〉0．05）；Bcl-2mRNA表达组间比较差异有统计学意义（F=19．87，P〈0．05），其中2、5、10μmol／LNaAs02染毒组Bcl-2mRNA表达（0．65±0．03、0．49±0．04、0．57±0．09）均低于对照组（0．95±0．11，P均〈0．05）；Bax／Bcl-2mRNA表达比值组间比较差异有统计学意义（F=8．35，P〈0．05），其中5、10μmol／LNaAsO2染毒组的Bax／Bcl-2mRNA表达比值（1.80±0．72、1．82±0．36）高于对照组（1．02±0．24，P均〈0．05）。结论NaAsO2可能通过降低PC12细胞PEDFmRNA的表达来增加凋亡相关因子Bax／Bcl-2mRNA表达．继而引起细胞凋亡。
Objective To observe the effects of different levels of sodium arsenite （NaAsO2） on mRNA expression of pigment epithelium-derived factor （PEDF） and apoptosis-related factors in PC12 cells （rat neuron properties pheochromocytoma）. Methods PC12 cells were treated with different levels of NaAsO2 [0 （control group）, 2, 5, 10 μmol/L] for 24 hours. The mRNA expression of PEDF and apoptosis-related factors （Bax, Bcl-2） were detected by real-time quantitative PCR. Results There were significant differences in the mRNA expressions of PEDF between the 4 groups （F = 102.28, P 〈 0.05）, the mRNA expressions of PEDF in the group of 2, 5, 10 μmol/L （0.70 ± 0.07, 0.33 ± 0.04, 0.23 ± 0.10） was lower than that of control group （1.15 ±0.11, P 〈 0.05）; there were no significant differences in the mRNA expressions of Bax between the 4 groups （0, 2, 5, 10 μmol/L groups： 0.95 ± 0.12, 0.80 ± 0.11, 0.88 ± 0.11, 1.01 ± 0.11, F = 2.01, P 〉 0.05）; there were significant differences in the mRNA expressions of Bcl-2 between the 4 groups （F = 19.87, P 〈 0.05）, the mRNA expressions of Bcl-2 in the group of 2, 5, 10 μmol/L （0.65 ± 0.03, 0.49 ± 0.04, 0.57 ± 0.09） were lower than that of control group （0.95 ± 0.11, all P 〈0.05）; there were significant differences in the mRNA expressions of Bax/Bel-2 between the 4 groups （F = 8.352, P 〈 0.05）, the mRNA expressions of in the group of 5, 10 μmol/L （1.80 ± 0.72, 1.82 ± 0.36） were higher than that of control group （1.02± 0.24, all P 〈 0.05）. Conclusion NaAsO2 may increase the expression of apoptosis-related factorsBax/ Bcl-2 mRNA by decreasing the expression of PEDF mRNA in PC12 cells, leading to apoptosis in PC12 cells.
出处
《中华地方病学杂志》
CAS
CSCD
北大核心
2016年第1期10-13,共4页
Chinese Journal of Endemiology
基金
高等学校博士学科点专项科研基金（20112307130003）
