新疆巴州地区布鲁氏菌流行株分子生物学鉴定

Molecular Biological Identification of the Epidemiological Strains of BrucellaIn Xinjiang

为新疆制定布病综合性防控措施提供科学依据和技术支撑,本研究应用分子生物学方法辅助鉴定了新疆巴州地区畜间布病流行分离株。2014年从新疆巴州地区收集流产羊胎儿21份,体内分离出疑似布鲁氏菌8株,采用分子分型方法(AMOS-PCR)和生物学分型方法对分离株进行进一步鉴定,结果表明8株分离株均为羊种布鲁氏菌,传统细菌分类学鉴定8株布鲁氏菌均为羊种生物3型。应用AMOS-PCR方法可以安全、快速对布鲁氏菌流行株进行种型鉴定,以期为新疆布病的综合防控措施的制定提供科学依据。

In order to formulate comprehensive measures of prevention and control brucellosis and provide the scientific ba- sis and technical support, the molecular biology method to identify epidemiological strains of livestock brucellosis with auxiliary was used in Bazhou area of Xinjiang in this study. In 2014, 21 aborted fetuses were collected from Bazhou in Xinjiang, 8 strains of suspected Brucella were isolated from the fetuses, and molecular classification method （AMOS-PCR） and biological typing method were used to further identify the isolates. The results showed that 8 isolated strains were identified as Brucella melitensis, 8 strains of Brucella identified as Brucella mclitensis biovar 3 by traditionaly bacterial taxonomy. Using AMOS-PCR method can safely and lastly identify the types of epidemiological strains of Brucella. This study can provide the scientific basis for the establishment of comprehensive measures of prevention and control brucellosis in Xinjiang.