野黄芩苷对人舌鳞癌Tca8113细胞Fas和FasL表达的影响

EFFECTS OF SCUTELLARIN ON EXPRESSION OF FAS AND FASL IN HUMAN TONGUE SQUAMOUS CARCINOMA TCA8113 CELLS

目的:观察野黄芩苷对人舌鳞癌Tca8113细胞Fas和Fas L表达的影响。方法:体外培养人舌鳞癌Tca8113细胞分为对照组、不同浓度野黄芩苷药物组,野黄芩苷组分别用80μg/ml、120μg/ml、160μg/ml的野黄芩苷作用人舌鳞癌Tca8113细胞48h。采用免疫细胞化学法观察野黄芩苷对人舌鳞癌Tca8113细胞Fas和FasL表达的影响。结果:用药组Tca8113细胞Fas和FasL的表达水平明显高于对照组(P<0.05);并且随着野黄芩苷药物浓度的增加,Fas和FasL的表达水平逐渐升高(P<0.05)。结论:野黄芩苷可能通过促进人舌鳞癌Tca8113细胞Fas蛋白和FasL蛋白的表达促进肿瘤细胞凋亡。

Objective：To observe the effects of scutellarin on expression of Fas and FasL in human tongue squamous carcinoma Tca8113 cells.Methods：Human tongue squamous carcinoma Tca8113 cells were cultured in vitro and divided into control group and 3 scutellarin groups with different concentrations（80μg/ml,120μg/ml,160μg/ml）.Immunocytochemical stain was used to detect the expression of Fas and FasL in human tongue squamous carcinoma Tca8113 cells.Results：The Fas and FasL expression level in Tca8113 cells of scutellarin groups was obviously higher than that of control group（P〈0.05）;Moreover,with increasing of scutellarin concentrations,the Fas and FasL expression increased gradually（P〈0.05）.Conclusions：Scutellarin may promote tumour cells apoptosis by upregulating the expression of Fas and FasL in human tongue squamous carcinoma Tca8113 cells.