英蔻化浊解毒方对胆汁反流性胃炎大鼠TNF-α表达的影响

Effect of yingkou huazhuo jiedu formula on tumor necrosis factor-αexpression of rats with bile reflux gastritis

目的:探讨中药英蔻化浊解毒方对胆汁反流性胃炎大鼠肿瘤坏死因子(TNF-α)表达的影响。方法:将60只大鼠随机分成4组,分别为空白对照组、模型组、西药组、英蔻化浊解毒方组,每组15只。采用以反流液空腹灌胃的方法制造胆汁反流性胃炎大鼠模型,各组大鼠均灌胃给药,西药组给予吗丁啉蒸馏水混悬液,中药组给予英蔻化浊解毒方混悬液,其余各组给予蒸馏水,均为每次1.5 m L,每日1次。灌胃给药时间从造模第2周开始,至第5周结束,连续4周。5周后将大鼠处死,取大鼠血清检测TNF-α含量,比较各组大鼠血清TNF-α含量的差异。结果:中药组与模型组比较有统计学意义(P<0.05),与西药组比较有统计学意义(P<0.05)。结论:英蔻化浊解毒方治疗胆汁反流性胃炎疗效确切,且治疗效果优于西药,其作用机制可能与降低血清中TNF-α含量有关。

Objective： To investigate the effect of yingkou huazhuo jiedu on the tumor necrosis factor-α（ TNF-α） expression of rats with bile reflux gastritis. Methods： 60 rats were randomly divided into four groups,namely blank control group,model group,western medicine group and yingkou huazhuo jiedu group,with 15 in each group. The models of rats with bile reflux gastritis were made by gavaging with the reflux liquid in a fasting state.The rats in each group were administered by gavage. The western medicine group was given the distilled water suspension of domperidone; and the Chinese drugs group was given the suspension of yingkou huazhuo jiedu; others group were given distilled water; all groups were gavaged 1. 5m L /1 time / per day. The gavage was lasted for four weeks,and it began from the first two weeks of modeling and ended at the fifth week. After 5 weeks,the rats were sacrificed; then their serum were taken,so as to detect the levels of TNF-α and compare the TNF-α differences of each group. Results： The TNF-α of yingkou huazhuo jiedu group was（ 0. 53 ± 0. 24） ng / m L,the model group was（ 0. 97 ± 0. 50） ng /m L,the differences between the two group were statistically significant（ P〈0. 05）; the TNF-αof western medicine group was（ 0. 90 ± 0. 31） ng / m L,compared with yingkou huazhuo jiedu group,the results were statistically significant（ P〈0. 05）. Conclusion： Yingkou huazhuo jiedu formula for treating bile reflux gastritis has better effect than western medicine. Their mechanism may be related to decreasing the content of serum TNF-α.