CIK/D-CIK治疗胃肠道肿瘤患者对免疫功能和近期疗效的评价

The evaluation on the changed to immune function and clinical symptoms for CIK / D- CIK treatment gastrointestinal tumor

目的:探讨CIK/D-CIK治疗胃肠道肿瘤患者对免疫功能的影响及临床疗效。方法:收集深圳市第二医院2013年6月—2015年1月胃肠道肿瘤行CIK/D-CIK治疗患者143例,对治疗前后血淋巴细胞亚群和肿瘤相关标志物进行统计分析,观察治疗前后血CD3^+CD45^+总T淋巴细胞、CD3^+CD4^+CD25^+辅助T淋巴细胞、CD3^+CD8^+CD28-抑制型T淋巴细胞、CD3^+CD8^+CD28^+活化型杀伤T淋巴细胞比例及相关性,以及癌胚抗原、CA125及CA199等相关肿瘤标志物比例变化。结果:经CIK/D-CIK治疗后患者临床症状改善,免疫功能增强,CD3^+CD45^+总T淋巴细胞、CD3^+CD8^+CD28-抑制型T淋巴细胞及CD3^+CD8^+CD28^+活化型杀伤T淋巴细胞比例较治疗前显著增高,CD3^+CD4^+CD25^+辅助T淋巴细胞比例降低,并与CD3^+CD8^+CD28-抑制型T淋巴细胞、CD3^+CD8^+CD28^+活化型杀伤T淋巴细胞比例呈负相关性,P<0.01(双侧),差异有统计学意义。癌胚抗原、CA125及CA199较治疗前显著降低,P<0.01(双侧),差异有统计学意义。结论:CIK/D-CIK可以显著提高胃肠道肿瘤患者免疫功能,改善临床症状,能有效抑制胃肠道肿瘤的复发和转移。

Objective： To discuss the changed to immune function and clinical symptoms for CIK / D- CIK treatment gastrointestinal tumors. Methods： Statistical analysis the group of blood lymphocytes and tumor related markers that collection of143 gastrointestinal tumor patients before or after treatment with CIK / D- CIK from June,2013 to January,2015 in shen zhen second hospital. To observe the blood CD3~＋CD45~＋T lymphocytes,CD3~＋CD4~＋CD25~＋T helper lymphocytes,CD3~＋CD8~＋CD28-conservation of T lymphocytes,CD3~＋CD8~＋CD28~＋activation type of destruction T lymphocytes proportion and correlation and the changed of CEA,CA125,CA199 and so on tumor related markers proportion after CIK / D- CIK treatment. Results： The patients＇ clinical symptoms changed and immune function enhanced and CD3~＋CD45~＋T lymphocytes,CD3~＋CD8~＋CD28-conservation of T lymphocytes,CD3~＋CD8~＋CD28~＋activation type of destruction T lymphocytes proportion significantly higher after CIK / D- CIK treatment than before CIK / D- CIK treatment,and CD3~＋CD4~＋CD25~＋T helper lymphocytes proportion reduce and negative correlation between CD3~＋CD8~＋CD28-conservation of T lymphocytes and CD3~＋CD8~＋CD28~＋activation type of destruction T lymphocytes,P〈0. 01（ both sides）,the differences was statistically significant. The CEA,CA125 and CA199 proportion has significantly reduce than before treatment with CIK / D- CIK,P〈0. 01（ both sides）,the differences was statistically significant. Conclusion： CIK / D- CIK can significantly enhance gastrointestinal tumor patients＇ immune function and changed clinical symptoms and effectively restrain gastrointestinal tumors recurrence and metastasis.