期刊文献+
任意字段
题名或关键词
题名
关键词
文摘
作者
第一作者
机构
刊名
分类号
参考文献
作者简介
基金资助
栏目信息

农村散养肉猪口蹄疫免疫抗体水平调查报告

下载PDF
导出
摘要 为了解农村散养肉猪口蹄疫免疫抗体水平情况,采集桂林市5个县(分别标注为A、B、C、D、E县)40个农村散养肉猪的血清样本进行检测。检测方法是用液相阻断酶联免疫吸附法,免疫抗体效力评价办法采用口蹄疫结构蛋白抗体滴度,本次调查每个县采集8个农村散养户,每户采40份肉猪血清样本。本次调查共采集肉猪血清样本1 600份。结果:A县有157份血清样本抗体滴度≥1∶64、163份≤1∶64;B县有139份血清样本抗体滴度≥1∶64、181份≤1∶64;C县有196份血清样本抗体滴度≥1∶64、124份≤1∶64;D县有168份血清样本抗体滴度≥1∶64、152份≤1∶64;E县有187份血清样本抗体滴度≥1∶64、133份≤1∶64。从调查结果不难看出,C县肉猪的免疫状态是最好的,但是从总体来看,免疫效果不容乐观。在抽检的1 600份血清中抗体滴度达到1∶64以上的仅仅有847份,免疫抗体滴度合格率仅仅为52.9%,未达到农业部的要求。
作者 朱春刚 尹丽萍
机构地区 广西大学动物科学技术学院 桂林市动物疫病预防控制中心 平乐县动物疫病预防控制中心
出处 《广西畜牧兽医》 2016年第1期17-18,共2页 Guangxi Journal of Animal Husbandry & Veterinary Medicine
关键词 散养肉猪 口蹄疫 抗体滴度 液相阻断酶联免疫吸附法
分类号 S858.282.43 [农业科学—临床兽医学]
  • 相关文献

参考文献8

  • 1OIE. Manual of Standards for Diagnostic Tests and Vac- cines[ z]. 2000,4th edition.
  • 2SAMUEL A R. KNOWLES N J, Foot-and-mouth disease type o virus exhibit genetically and geographically distinct volutionary lineages (topotypes) [ J ]. J Gen Virol. 2001,82 (3) :609 -621.
  • 3杨苏珍,卢曾军,曹轶梅,李冬,刘在新,贾宁.牛、羊、猪人工感染口蹄疫病毒3ABC抗体的变化[J].甘肃农业大学学报,2006,41(5):22-26. 被引量：14
  • 4PAIBA G A,ANDERSON J,PATON D J, et al. Validation of a foot-and-mouth disease antibody screening solid-phase corn-petition EILSA (SPCE) [ J J. J Virol Methods. 2004, 115(2) :145 -158.
  • 5MA Jun-wu, LIU Xiang-tao, HU Hong-bo, et al. A liquid phase blocking enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies against foot-and- mouth disease virus [ C ]//The Branch of Foot-and-Mouth Disease Science of Chinese Association of Animal Science and Veterinary Medicine. The proceedings of the 9th na- tional conference on foot-and--mouth disease sponsored by the Branch of Foot-and-Mouth Disease Science of Chinese Association of Animal Science and Veterinary Medicine. Lanzhou: Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, 2003 : 364 - 368. ( in Chinese).
  • 6H AM BLIN C. BARNET I T R . H EDGER R S. A new enzyme-linked immunosorbent assay (ELISA) for the detec- tion of anti-bodies against foot-and-mouth disease virus. I. Development and method of ELISA [ J ]. J Immnnol Meth- ods, 1986,93 (1) : 115 - 121.
  • 7HAM BI IN C. BARNET I T R, HEDGER R S. A new en- zyme-linked immunosorbent assay ( ELISA ) for the detec- tion of anti-bodies against foot-and-mouth disease virus. 11. Application[ ~ ]. J Immunol Methods, 1986,93 ( 1 ) : 123 - 129.
  • 8MA Jun-wu, LIN Mi, QI SHU-yun, et al. A liquid-phase blocking enzyme-linked immunosorbent assay ( ELISA ) for the detection of specificity and sensibility against foot-and- mouth disease virus type Asia I [ J ~. Chinese Journal of Veterinary Science and Technology, 2005,35 ( 2 ) : 378 - 380. (in Chinese).

二级参考文献10

  • 1吴锦艳,刘湘涛,胡永浩,尚佑军,田宏,孙世琪,尹双辉,冯霞.猪口蹄疫病毒对三种动物细胞嗜性的研究[J].甘肃农业大学学报,2005,40(3):306-310. 被引量：8
  • 2Pinto A A,Garland A J.Immune response to virus-infection-associated (VIA) antigen in cattle repeatedly vaccinated with foot-and-mouth disease virus inactivated by formalin or acetylethyleneimine[J].J Hyg,1979,82:41～50
  • 3Alonso A,Gomes M P D,Martins M A,et al.Detection of foot-and-mouth disease virus infection-associated antigen antibodies:comparison of the enzyme-linked immunosorbent assay and agar gel immunodiffusion tests[J].Prev Vet Med,1990,(9):233～240
  • 4Diego D M,Brocchi E,Mackay D,et al.The non-structural polyprotein 3ABC of foot-and-mouth virus as a diagnostic antigen in ELISA to differentiate infected from vaccinated cattle[J].Arch Virol,1997,142:2021～2033
  • 5Bergmann I E,Mello P A,de Neitzert E,et al.Diagnosis of persistent aphthovirus infection and its differentiation from vaccination response in cattle by use of enzyme-linked immunoelectrotransfer blot analysis with bioengineered nonstructural viral antigens[J].Am J Vet Res,1993,54(6):825～831
  • 6Rodriguez A,Dopazo J,Saiz J C,et al.Immunogenicity of non-structural proteins of foot-and-mouth disease virus:differences between infected and vaccinated swine[J].Arch Virol,1994,136:123～131
  • 7Lubroth J,Brown F.Identification of native foot-and-mouth disease virus non-structural protein 2C as a serological indicator to differentiate infected from vaccinated animals[J].Res Vet Sci,1995,59:70～78
  • 8Newman J F E,Cartwright B,Doel T R,et al.Purification and identification of the RNA-dependent polymerase of foot-and-mouth disease virus[J].J Gen Virol,1979,45:497～507
  • 9Polatnick J,Arlinghaus J H,Cowan K M.Inhibition of cell-free foot-and-mouth disease virus-ribonucleic acid synthesis by antibody[J].Virology,1967,31:609～615
  • 10Lubroth J,Lope z A,Ramalho A K,et al.Cattle response to foot-and-mouth disease virus nonstructure proteins as antigens within vaccines produced using different concentrations[J].Vet Q,1998,20(2):13～15

共引文献13

广西畜牧兽医
2016年 第1期

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部