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Wnt/β-catenin通路激动剂-LiCl对3T3-L1细胞Villin 2表达的影响

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摘要 试验旨在探究Wnt/β-catenin通路激动剂-Li Cl对3T3-L1细胞Villin 2表达的影响,明确Villin 2在脂肪生成中是否与Wnt/β-catenin信号通路相关。培养3T3-L1细胞,分别设置对照组和20 m M Li Cl处理组,油红O染色观察3T3-L1细胞分化的形态变化;荧光定量(PCR)检测Villin 2 mRNA水平的变化。结果表明:20 m M Li Cl处理组相对于对照组显著抑制3T3-L1细胞分化;在3T3-L1细胞分化过程中和完全分化后20 m M Li Cl处理组的Villin 2 mRNA相对表达量都显著高于对照组(P<0.05)。因此,20 m M Li Cl能在3T3-L1细胞中成功激活Wnt/β-catenin信号通路,Villin 2在3T3-L1细胞分化过程中和完全分化后都是作为一个下调基因起抑制脂肪生成的作用,并且Villin 2与Wnt/β-catenin信号通路相关。
出处 《饲料研究》 CAS 2016年第1期52-56,共5页 Feed Research
基金 黑龙江省普通高等学校饲料营养重点实验室开放课题资助项目(yy-2012-06) 高等学校博士学科点专项科研基金(20130061120112)
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