摘要
通过紫外-可见分光光度法测定了碳酸镧吸附前后溶液中的DNA含量,并计算了DNA的吸附率.通过X射线光电子能谱(XPS)与X射线衍射(XRD)分析了吸附DNA前后固相的变化.结果表明,碳酸镧对DNA有较强的吸附作用,且该过程对碳酸镧晶型未产生明显影响.通过对吸附条件的考察,发现DNA的吸附率随pH值升高而降低.最后通过磷酸二氢钾作为洗脱液将吸附的DNA回收,洗脱液中镧离子含量低于0.1×10^(-6)mol/L,对DNA的后期使用无污染.
The adsorption behavior of Deoxyribonucleic acid（ DNA） on lanthanum carbonate particles was investigated. Both UV and XPS results demonstrated that DNA could be adsorbed on the surface of lanthanum carbonate particles. Moreover,the efficiency of adsorption was maximized when the p H value of the solution was set around 5. 5. In addition,DNA could be desorpted when lanthanum carbonate particles with adsorpted DNA was dispersed in aqueous solution of KH2PO4. The ICP results demonstrated that the concentration of La3 ＋was lower than 0. 1 μmol / L in the aqueous solution after desorption process,thereby excluding the possibility of undesirable influence on DNA.
出处
《高等学校化学学报》
SCIE
EI
CAS
CSCD
北大核心
2016年第1期7-11,共5页
Chemical Journal of Chinese Universities
基金
国家自然科学基金(批准号:51373003
30873437)
教育部博士点基金(批准号:20112133110001)资助~~
关键词
脱氧核糖核酸
碳酸镧
富集
脱附
Deoxyribonucleic acid（DNA）
Lanthanum carbonate
Enrichment
Desorption
