麦草畏、水杨酸对西洋参成熟种胚体细胞胚发生的影响

Effect of Dicamba and Salicylic Acid on Somatic Embryogenesis of American Ginseng Mature Embryo(Panax quinquefolium)

以西洋参成熟种胚为试材,以不加激素和添加0.5mg/L 2,4-D的MS培养基为对照,研究了不同浓度（0.05-2.00mg/L）麦草畏、水杨酸对西洋参体胚发生的影响。结果表明：未添加激素的空白培养基没有愈伤和体胚发生;在附加2,4-D的培养基中愈伤和体胚发生率为100%;5个浓度的麦草畏和水杨酸均能诱导出体胚,其中1.00mg/L麦草畏的效果最佳,体胚发生率为56.60%,水杨酸处理下体胚发生率最高为7.50%;在各种浓度的水杨酸中种胚生长量比对照小且褐化严重。说明麦草畏、水杨酸均可诱导西洋参成熟种胚体细胞胚发生,诱导能力2,4-D〉麦草畏〉水杨酸;水杨酸对种胚生长有抑制作用。

Taking mature seed embryo of American Ginseng（Panax quinquefolium）as materials,using MS basal medium and MS＋0.5 mg/L 2,4-D as controls,the effect of dicamba and salicylic acid（0.05-2.00 mg/L）on somatic embryogenesis of American Ginseng mature embryo was researched.The results showed that on MS basal medium without any hormones there were no callogenesis and embryogenesis;on MS＋0.5mg/L 2,4-D,the percentage of both attained to 100%;medium with five concentration of dicamba and salicylic acid all could induce somatic embryogenesis,in which the effect of 1.00mg/L dicamba was the best,embryogenesis rate reached 56.60%,and the highest of salicylic acid was 7.5%;in salicylic acid,the growth volume of embryo was smaller than that of controls.The experiment showed that dicamba and salicylic acid could induce somatic embryogenesis on mature seed embryo of American Ginseng,induction capacity was 2,4-Ddicambasalicylic acid;salicylic acid showed inhibitory effect on embryo growth.