火龙果花中黄酮类化合物抗氧化活性研究

Study on the Antioxidative Activity of Flavonoids From Pitaya Flower

为了寻找安全的天然抗氧化剂,以火龙果花为研究对象,以乙醇作为提取溶剂,对火龙果花进行加热回流提取,以体外抗氧化试验为活性评价手段,测定其中的总黄酮含量,利用DPPH法、水杨酸法、邻苯三酚法、硫氰酸铁法、普鲁士蓝法从5个方面测定样品的抗氧化活性,以化学合成的脂溶性抗氧化剂TBHQ为阳性对照品。研究不同浓度下火龙果花中黄酮类化合物的抗氧化活性。结果表明:火龙果花总黄酮含量为2.62mg/mL,黄酮类化合物对DPPH·的清除能力与特丁基对苯二酚(TBHQ)相当,清除·OH的IC50为0.57mg/mL,清除超氧阴离子的IC50为1.41mg/mL,抑制脂质过氧化的IC50为1.80mg/mL,还原力相对较弱。研究表明火龙果花中黄酮类化合物具有较强的抗氧化活性,可作为天然抗氧化剂推广应用。

In order to find safe natural antioxidant,taking pitaya flower as the research object,with ethanol as extraction solvent,heating reflux extraction of pitaya flower for in vitro antioxidant activity evaluation method,total flavonoids content was determined,antioxidant activity of samples was determined from five aspects,using the method of DPPH,salicylic acid,adjacent phenyl phenol method,ferric thiocyanate method,Prussian blue method,with chemical synthetic fat-soluble antioxidants TBHQ as positive reference substance,the pitaya flower under different concentrations of antioxidant activity of flavonoids was researched.The results showed the pitaya flower flavonoids content was 2.62mg/mL,removal ability of flavonoids on DPPH · was similar with TBHQ,IC50 of clearing·OH was 0.57mg/mL,IC50 of removing superoxide anion was 1.41mg/mL,IC50 of inhibiting of lipid peroxidation was 1.80mg/mL,reducing power was relatively weak.Studies showed that flavonoids pitaya flower had strong antioxidant activity,as the application of natural antioxidants.