大豆MYB转录因子的全基因组鉴定及生物信息学分析

Whole Genome Identification of Soybean MYB Transcription Factors and Bioinformatics Analysis

MYB转录因子是植物中最大的转录因子家族之一,近年的研究表明其广泛参与多种生物学过程。MYB转录因子在其N端含有其特有的DNA结合基序(MYB-binding domain),能够与DNA分子大沟结合而调控基因表达。本研究通过生物信息学手段,在全基因组水平上筛选鉴定出了304个大豆MYB类转录因子,并对其进行了分类和保守结构域分析;通过与拟南芥的MYB基因进行系统发生分析,将304个大豆MYB转录因子分为了22个亚类;染色体定位分析表明大豆MYB转录因子在所有染色体上都有分布,部分染色体间的MYB蛋白序列高度相似,表明此类基因在进化上具有相同的来源;利用野生大豆盐、碱胁迫下转录组数据,分析MYB基因的表达模式,发现部分MYB转录因子能够响应盐、碱胁迫的诱导,且在盐、碱胁迫下具有不同的表达模式。

Key Laboratory of Agricultural Biological Functional Genes, Northeast Agricultural University, Harbin 150030, ChinaAbstract： MYB transcription factor family is one of the largest families of transcription factors in plants.Recent research indicated MYB transcription factors participate in variety kinds of biological processes. MYB transcription factors have a unique DNA-binding domain in their N terminal, which can combine with DNA molecules and regulate the expression of downstream genes.In this study we conducted a genome-wide analysis to identify, classified 304 MYB transcription factors of soybean and analyzed their conserved domain.These MYB genes were classified into 22 subgroups by phylogenetic analysis. Chromosomal location analysis was conducted. Further analysis showed that part of the MYB genes among different chromosomal showed highly similarity. Transcriptome analysis of the MYB genes showed that most of them could response to salinity-alkalinity stress.