干旱胁迫下DCPTA对大豆幼苗生长的调控

Regulation of DCPTA on Soybean Seedling Growth under Drought Stress

以嫩丰20为试验材料,在温室和盆栽条件下,以清水为对照,用15%的PEG-6000模拟干旱条件,设置4个浓度(0,20,40和60 mg·L^(-1))的DCPTA溶液处理进行叶面喷施,考察干旱胁迫下其对大豆幼苗叶片光合特性、保护酶以及叶绿素荧光特性的影响。结果表明:DCPTA处理提高了苗期大豆叶片光合速率、蒸腾速率、气孔导度和叶绿素含量,降低了胞间CO_2浓度;提高了最大荧光量(Fm)、PSⅡ的最大光能转换速率(Fv/Fm)和光化学淬灭系数(q P);降低了非光化学淬灭系数(q N);增加了保护酶活性;并促进了脯氨酸的积累。过氧化氢酶(CAT)活性和脯氨酸含量以60 mg·L^(-1)为最优处理,其余指标均以40 mg·L^(-1)处理为最优。

The objective of this study is to determine the mechanisms of soybean seedlings leaf photosynthetic characteristics, protective enzymes and chlorophyll fluorescence characteristics regulation under drought resistance by foliar application of tertiary amine active DCPTA. Nenfeng 20 growing in pot under greenhouse was treated by 15% PEG-6000 to simulate drought conditions. Four different concentrations were used as treatments, including 0, 20, 40 and 60 mg.L-1and water was used as control. The result indicated that the photosynthetic rate, transpiration rate, stomatal conductance and chlorophyll content, as well as .Fm, Fv/Fm and qP increased significantly while the intercellular CO2concentration and qNdecreased after DCTA treatment.DCTA also promoted the activity of protective enzymes and the accumulation of proline in soybean leaves 60 mg.L-1was the most optimized concentration of CAT and proline, while 40 mg？L-1？was the optimum treatment for other parameters.