大豆细菌性病害病原菌分离鉴定与致病性评价

Isolation,Identification and Pathogenicity Evaluation of Soybean Bacterial Disease Pathogens

对在黑龙江哈尔滨闫家岗农场采集的绥农14大豆细菌性斑点病叶片进行病原菌分离,将分离得到的大豆细菌性斑点病病原菌进行抗生素耐性鉴定、分子鉴定并对大豆品种回接试验,分离得到1个细菌菌株。经过革兰氏染色、病原菌回接试验、16S r DNA分子鉴定方法确定分离的菌株为丁香假单胞杆菌属,命名为Psgneau 2。分离的菌株对终浓度为25,50,100μg·m L^(-1)的氨苄青霉素和羧苄青霉素都具有耐性,对壮观青霉素、利福平、卡那霉素、庆大霉素、四环素和氯霉素不具有抗性。采用高压喷雾和菌落滴度的方法将分离的Psgneau 2菌株接种10个大豆品种,进行致病性鉴定,结果表明绥农14极易感病,合丰35抗病性高于其它品种。

In order to research soybean bacterial disease in Heilongjiang province, one bacterial pathogen was isolated from Suinong 14 diseased leaves which were collected from Harbin Yanjiagang farm. The pathogen has been carried out antibiotic resistance identification, molecular identification, re-inoculated identification and pathogenicity identification. By Gram staining, pathogen-back test, 16S rDNA molecular identification, the pathogen was Pseudomonas syringae and named Psgneau 2. The pathogen exhibited resistance against two antibiotics, ampicillin and carbenicillin, as three concentration 25，50，100 μg·mL-1, but susceptible to spectinomycin, rifampicin, kanamycin, gentamicin, tetracycline and chloramphenicol. Ten soybean varieties were inoculated with Psgneau 2, of which Suinong 14 was more susceptible than others and Hefeng 35 was the most resistant to Psgneau 2.