大豆四向重组自交群体单株产量QTL单标记分析

Single Marker Analysis on QTL Conditioning Yield per Plant in Soybean by Four-way Recombinant Inbred Lines Population

以（垦丰14×垦丰15）×（黑农48×垦丰19）衍生的含160个株系的大豆四向重组自交系群体为试验材料，应用154个SSR标记鉴定个体基因型，利用单标记分析方法，对2013和2014年在哈尔滨和克山两地4个环境下的单株产量进行QTL定位分析。结果显示：共检测到46个与单株产量相关的QTL位点，主要分布在A1、A2、C1、C2、D2、D1b、L、K、B2、N、E、J、F、G和H连锁群上，遗传率为0.15%~9.37%。遗传率较高的标记位点有BARCSOYSSR_02_0607、BARCSOYSSR_03_1620、BARCSOYSSR_19-0451、Sat_153、Sat_367、Satt229和Satt529，优异等位基因型为BARCSOYSSR_02_0607（Q1Q1）、BARCSOYSSR_03_1620（Q2Q2）、BARCSOYSSR_09_0183（Q1Q1）、Satt229（Q2Q2）、Sat_367（Q3Q3）、Satt338（Q1Q1）、Satt229（Q1Q1）和Satt668（Q1Q1）。在检测的标记位点中，BARCSOYSSR_08_0966、Sat_36、Sat_153、BARCSOYSSR_02_0607和Satt529在两个环境中重复检测，表明这5个QTL可用于分子设计育种改良单株产量。

In this paper a four-way recombinant inbred lines （FWRIL） population, which came from double cross （Kenfeng 14×Kenfeng 15） ×（Heinong 48×Kenfeng 19） through continuous self-fertilizer 6 times and was composed of 160 lines, was used to map QTL conditioning yield per plant （YPP） based the genotypic data came from 154 SSR primers and phenotypic data of YPP came from 4 planting environments, i.e. Keshan in 2013, Harbin in 2013, the first sowing date of Harbin in 2014, the second sowing date of Harbin in 2014, by single marker analysis method.The results showed that 46 QTLs underlying YPP were detected significantly and the QTLs distributed on linkage group A1, A2, C1, C2, D2, D1b, L, K, B2, N, E, J, F, G and H with the range of heritability from 0.15%-9.37%.The QTLs showed higher heritability including BARCSOYSSR_02_0607, BARCSOYSSR_03_1620, BARCSOYSSR_19-0451, Sat_153, Sat_367, Satt229 and Satt529. The excellent allele genotypes that could increase YPP under its QTL include BARCSOYSSR_02_0607（Q1Q1）, BARCSOYSSR_03_1620（Q2Q2）, BARCSOYSSR_09_0183（Q1Q1）, Satt229（Q2Q2）, Sat_367（Q3Q3）, Satt338（Q1Q1）, Satt229（Q1Q1） and Satt668（Q1Q1）.Among all detected QTLs, BARCSOYSSR_08_0966, Sat_36, Sat_153, BARCSOYSSR_02_0607 and Satt529 are mapped tautologically in two environments, which indicates these five QTLs could be utilized in molecular design breeding to improve YPP.