嫌色性肾细胞癌和肾嗜酸细胞腺瘤中CK7、Claudin-7、Epcam、vimentin的表达及其临床意义

Expression of CK7,Claudin-7,Epcam,vimentin in chromophobe renal cell carcinoma and renal oncocytoma and their significance

目的探讨CK7、Claudin-7、上皮细胞黏附分子(Epcam)和vimentin在嫌色性肾细胞癌(chromophobe renal cell carcinoma,Ch RCC)和肾嗜酸细胞腺瘤(renal oncocytoma,RO)中的表达,及其诊断与鉴别诊断的意义。方法采用免疫组化En Vision两步法检测24例Ch RCC和17例RO中CK7、Claudin-7、Epcam和vimentin的表达。结果 CK7、Claudin-7、Epcam和vimentin在Ch RCC中的阳性率分别为79.17%(19/24)、95.83%(23/24)、91.67%(22/24)、8.33%(2/24),在RO中的阳性率分别为23.53%(4/17)、41.18%(7/17)、29.41%(5/17)、0(0/17);CK7、Claudin-7和Epcam在这两种肿瘤中的表达差异有统计学意义(P<0.05);虽然vimentin在两种肿瘤中的表达差异无统计学意义(P>0.05),但与其它含有嗜酸性细胞的肾脏肿瘤表达差异有显著性。结论 CK7、Claudin-7和Epcam可作为鉴别Ch RCC与RO的免疫标志物,vimentin有助于Ch RCC、RO与其它含有嗜酸性细胞的肾脏肿瘤鉴别。

Purpose To investigate the expression of CK7, Claudin-7, Epcam and vimentin in chromophobe renal cell carcinoma （ChRCC） and renal oncocytoma （RO） and their significance in diagnosis and differential diagnosis. Methods The expression of CKT, Claudin-7, Epcam and vimentin in 24 cases of ChRCC and 17 cases of RO were detected by immunohistochemieal EnVision two step method. Results The expression rates of CKT, Claudin-7, Epcam and vimentin in ChRCC were 79. 17% （19/24） , 95.83% （23/24）, 91.67% （22/24） and 8.33% （2/24） respectively, while in RO were 23.53% （4/17）, 41.18% （7/17）, 29.41% （5/ 17） and 0（0/17） , respectively. The expression of CK7, Claudin-7 and Epcam was different in ChRCC and RO and the results had significant significance （ P 〈 0. 05 ）. The expression of vimentin was indifferent between the two tumors and had no significant values （ P 〉 0. 05 ）, but vimentin had significant differences between the two and other renal tumors which contain eosinophilic cells. Conclusion CKT, Claudin-7 and Epcam may be the useful markers to discriminate between ChRCC and RO. Vimentin are helpful for distinguishing ChRCC and RO from other renal tumors which contain eosinophilic cells.