GOLPH3、VEGF在胃癌组织中的表达及其相互关系

Relationship between the expression of GOLPH3 with VEGF expression in gastric cancer

目的通过检测胃癌组织中高尔基体磷酸化蛋白3(GOLPH3)与血管内皮生长因子(VEGF)表达水平,探讨二者在胃癌进展中的相互关系。方法通过免疫组织化学SP法检测55例经手术切除的胃癌组织及相应癌旁组织中GOLPH3和VEGF蛋白的表达情况。GOLPH3与VEGF在胃癌组织中表达相关性采用Pearson相关性分析。结果GOLPH3及VEGF在胃癌组织中的阳性表达率分别为67.27%、58.18%,显著高于相应癌旁组织的18.18%、23.64%,差异有统计学意义(P<0.05)。GOLPH3、VEGF在胃癌组织中的表达与患者性别、年龄、肿瘤大小等均无显著相关性,与浸润深度、淋巴结转移和TNM分期有显著相关性(P<0.05)。免疫组织化学结果显示在GOLPH3阳性表达组的胃癌组织中,VEGF阳性表达率为75.68%,明显高于GOLPH3阴性表达组的22.22%,胃癌组织中GOLPH3表达与VEGF的表达呈正相关性(r=0.508,P<0.01)。结论GOLPH3过表达可能通过上调VEGF的表达调节胃癌血管生成,从而影响胃癌的浸润和转移。

Objective To investigate the interrelationship between Golgi phosphoprotein 3 （GOLPH3） expression with vasculoar endothelial growth factor（VEGF） expression in the progression of human gastric cancer by detecting the expression level of GOLPH3 and VEGF. Methods Immunohistoehemistry （IHC） was used to detect the ex- pression of GOLPH3 and VEGF in 55 cases of gastric cancerous and carcinoma-adjacent tissues. Pearson correlation analysis was used to discuss the association between GOLPH3 expression with VEGF expression. Results The im- munohistochemical detection showed that the positive expression rates of GOLPH3 and VEGF were 67.27%, 58. 18% respectively, which were significantly higher than the positive expression rates in carcinoma-adjacent tis- sues （P 〈0. 05）. A statistical analysis revealed that the positive expression rate of GOLPH3 and VEGF in the gas- tric cancer tissues were strongly correlated with depth of invasion （P 〈 0. 05 ）, distant metastasis （ P 〈 0.01 ） and TNM stages （ P 〈 0. 01 ） , whereas it did not significantly correlate with age or gender or size of tumor. Compared with the negative for the GOLPH3 expression, the VEGF expression in gastric cancer tissues positive for the GOL- PH3 expression was significantly higher. The protein level of GOLPH3 expression was positively correlated with VEGF expression （ r = 0. 508,P 〈0. 01 ）. Conclusion GOLPH3 overexpression may regulate gastric angiogenesis by upregulating the expression of VEGF, which affects the invasion and metastasis of gastric cancer.